Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 18062778
Iida M, Matsuda M, Komatani H (2008) Plk3 phosphorylates topoisomerase IIalpha at Thr(1342), a site that is not recognized by Plk1. Biochem J 411, 27-32 18062778
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

S133-p - CCNB1 (human)
Modsite: sPsPMETsGCAPAEE SwissProt Entrez-Gene
Orthologous residues
CCNB1 (human): S133‑p, CCNB1 (mouse): S130‑p, CCNB1 (rat): S123‑p

S147-p - CCNB1 (human)
Modsite: EDLCQAFsDVILAVN SwissProt Entrez-Gene
Orthologous residues
CCNB1 (human): S147‑p, CCNB1 (mouse): S144‑p, CCNB1 (rat): S137‑p

S198-p - CDC25C (human)
Modsite: sDELMEFsLKDQEAK SwissProt Entrez-Gene
Orthologous residues
CDC25C (human): S198‑p, CDC25C iso3 (human): S155‑p, CDC25C (mouse): S220‑p, CDC25C (frog): S270‑p

T1343-p - TOP2A (human)
Modsite: FsDFDEKtDDEDFVP SwissProt Entrez-Gene
Orthologous residues
TOP2A (human): T1343‑p, TOP2A iso2 (human): T1369‑p, TOP2A iso4 (human): T1424‑p, TOP2A (mouse): D1339‑p, TOP2A (rat): D1339‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK3 (human)
KINASE PLK1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PLK3 (human) transfection of dominant-negative enzyme, co-immunoprecipitation