Wang ZH, et al. (2017) Delta-Secretase Phosphorylation by SRPK2 Enhances Its Enzymatic Activity, Provoking Pathogenesis in Alzheimer's Disease. Mol Cell 67, 812-825.e5 28826672

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S226-p - LGMN (human) ▲

Modsite: CYYDEkRsTYLGDWY SwissProt Entrez-Gene Orthologous residues LGMN (human): S226‑p, LGMN iso3 (human): S226‑p, LGMN (mouse): G228‑p, LGMN (rat): S228‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293FT, brain, HeLa (cervical) Cellular systems studied:  cell lines, tissue Species studied:  human, mouse, rat Enzymes shown to modify site in vitro:  Type Enzyme KINASE SRPK2 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE SRPK2 (human) microscopy-colocalization, transfection of constitutively active enzyme, co-immunoprecipitation, transfection of wild-type enzyme Downstream Regulation Effect of modification (function):  enzymatic activity, induced Comments:  AD Pathologies in Young 3xTg mice, stimulating cognitive dysfunction Associated Diseases Diseases Alterations Comments Alzheimer's disease increased

T492-p - SRPK2 (human) ▲

Modsite: PSHDRSRtVsAsstG SwissProt Entrez-Gene Orthologous residues SRPK2 (human): T492‑p, SRPK2 iso2 (human): T503‑p, SRPK2 (mouse): T485‑p, SRPK2 (rat): T485‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293FT, brain, HeLa (cervical) Cellular systems studied:  cell lines, tissue Species studied:  human, mouse, rat Associated Diseases Diseases Alterations Comments Alzheimer's disease increased

S228-p - LGMN (rat) ▲

Modsite: CYYDEERsTYLGDWY SwissProt Entrez-Gene Orthologous residues LGMN (human): S226‑p, LGMN iso3 (human): S226‑p, LGMN (mouse): G228‑p, LGMN (rat): S228‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293FT, brain, HeLa (cervical) Cellular systems studied:  cell lines, tissue Species studied:  human, mouse, rat

T485-p - SRPK2 (rat) ▲

Modsite: PSRDRSRtVsASstG GenPept Entrez-Gene Orthologous residues SRPK2 (human): T492‑p, SRPK2 iso2 (human): T503‑p, SRPK2 (mouse): T485‑p, SRPK2 (rat): T485‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293FT, brain, HeLa (cervical) Cellular systems studied:  cell lines, tissue Species studied:  human, mouse, rat