Takada M, et al. (2017) FBW7 Loss Promotes Chromosomal Instability and Tumorigenesis via Cyclin E1/CDK2-Mediated Phosphorylation of CENP-A. Cancer Res 77, 4881-4893 28760857

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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S17-p - CENPA (human) ▲

Modsite: PEAPRRRsPsPtPtP SwissProt Entrez-Gene Orthologous residues CENPA (human): S17‑p, CENPA (mouse): P14‑p, CENPA (rat): P14‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  colorectal cancer, colorectal carcinoma Relevant cell lines - cell types - tissues:  DLD1 (intestinal), MCF10A1 (epithelial) Cellular systems studied:  cell lines Species studied:  human

S19-p - CENPA (human) ▲

Modsite: APRRRsPsPtPtPGP SwissProt Entrez-Gene Orthologous residues CENPA (human): S19‑p, CENPA (mouse): S16‑p, CENPA (rat): S16‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  colorectal cancer, colorectal carcinoma Relevant cell lines - cell types - tissues:  DLD1 (intestinal), MCF10A1 (epithelial) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE CDK2 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE CDK2 (human) siRNA inhibition of enzyme, transfection of wild-type enzyme, transfection of inactive enzyme Downstream Regulation Effect of modification (function):  intracellular localization, molecular association, regulation Effect of modification (process):  cell cycle regulation, cell growth, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays HJURP (human) Disrupts pull-down assay Comments:  localization to centromeres, tumor progression

T21-p - CENPA (human) ▲

Modsite: RRRsPsPtPtPGPsR SwissProt Entrez-Gene Orthologous residues CENPA (human): T21‑p, CENPA (mouse): A18‑p, CENPA (rat): A18‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  colorectal cancer, colorectal carcinoma Relevant cell lines - cell types - tissues:  DLD1 (intestinal), MCF10A1 (epithelial) Cellular systems studied:  cell lines Species studied:  human

T23-p - CENPA (human) ▲

Modsite: RsPsPtPtPGPsRRG SwissProt Entrez-Gene Orthologous residues CENPA (human): T23‑p, CENPA (mouse): ‑, CENPA (rat): A43‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  colorectal cancer, colorectal carcinoma Relevant cell lines - cell types - tissues:  DLD1 (intestinal), MCF10A1 (epithelial) Cellular systems studied:  cell lines Species studied:  human

S68-p - CENPA (human) ▲

Modsite: LIRKLPFsRLAREIC SwissProt Entrez-Gene Orthologous residues CENPA (human): S68‑p, CENPA (mouse): S62‑p, CENPA (rat): G87‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  colorectal cancer, colorectal carcinoma Relevant cell lines - cell types - tissues:  DLD1 (intestinal), MCF10A1 (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE CDK1 (human) siRNA inhibition of enzyme, transfection of wild-type enzyme, phospho-antibody