Curated Information
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Home > Curated Information Page > PubMed Id: 28756945
Shan P, et al. (2017) SIRT1 Functions as a Negative Regulator of Eukaryotic Poly(A)RNA Transport. Curr Biol 27, 2271-2284.e5 28756945
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S14-p - SIRT1 (human)
Modsite: LALQPGGsPsAAGAD SwissProt Entrez-Gene
Orthologous residues
SIRT1 (human): S14‑p, SIRT1 (mouse): S14‑p, SIRT1 (rat): S14‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Disease tissue studied:  bone cancer, colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal), HEK293T (epithelial), HeLa (cervical), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
glucose_starvation no change compared to control

T530-p - SIRT1 (human)
Modsite: YLSELPPtPLHVsED SwissProt Entrez-Gene
Orthologous residues
SIRT1 (human): T530‑p, SIRT1 (mouse): T522‑p, SIRT1 (rat): T524‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Disease tissue studied:  bone cancer, colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal), HEK293T (epithelial), HeLa (cervical), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
glucose_starvation increase
AMPKA1 (human) increase shAMPK decrease
Downstream Regulation
Effect of modification (function):  intracellular localization, molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
PABP 1 (human) Induces co-immunoprecipitation
Comments:  nuclear accumulation of both PABP1 and poly(A)RNA

T719-p - SIRT1 (human)
Modsite: AGGAGFGtDGDDQEA SwissProt Entrez-Gene
Orthologous residues
SIRT1 (human): T719‑p, SIRT1 (mouse): A709‑p, SIRT1 (rat): A710‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Disease tissue studied:  bone cancer, colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal), HEK293T (epithelial), HeLa (cervical), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
glucose_starvation increase