Curated Information
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Home > Curated Information Page > PubMed Id: 20362052
Faour WH, Thibodeau JF, Kennedy CR (2010) Mechanical stretch and prostaglandin E2 modulate critical signaling pathways in mouse podocytes. Cell Signal 22, 1222-30 20362052
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T308-p - Akt1 (mouse)
Modsite: KDGAtMKtFCGtPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'renal, podocyte'
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PGE2 decrease

S473-p - Akt1 (mouse)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'renal, podocyte'
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PGE2 decrease
H-89 PGE2 inhibit treatment-induced decrease
colforsin, IBMX decrease
H-89 colforsin, IBMX inhibit treatment-induced decrease
PGE2 PTGER4 (mouse) decrease PTGER4 siRNA inhibits decrease
glucose increase high glucose
PGE2 glucose inhibit treatment-induced increase
vanadate increase
PGE2 vanadate inhibit treatment-induced increase
H2O2 increase
PGE2 H2O2 inhibit treatment-induced increase
vanadate H2O2 augment treatment-induced increase
cyclic_stretch no change compared to control
H2O2 cyclic_stretch increase
vanadate cyclic_stretch, H2O2 augment treatment-induced increase

T203-p - ERK1 (mouse)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'renal, podocyte'
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H2O2 increase
cyclic_stretch increase
H2O2 cyclic_stretch no effect upon treatment-induced increase
low_glucose increase
glucose increase
cyclic_stretch low_glucose augment treatment-induced increase
cyclic_stretch glucose augment treatment-induced increase
AG1478 decrease
cyclic_stretch AG1478 inhibit treatment-induced decrease

Y205-p - ERK1 (mouse)
Modsite: HtGFLtEyVAtRWyR SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'renal, podocyte'
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H2O2 increase
cyclic_stretch increase
H2O2 cyclic_stretch no effect upon treatment-induced increase
low_glucose increase
glucose increase
cyclic_stretch low_glucose augment treatment-induced increase
cyclic_stretch glucose augment treatment-induced increase
AG1478 decrease
cyclic_stretch AG1478 inhibit treatment-induced decrease

T183-p - ERK2 (mouse)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'renal, podocyte'
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H2O2 increase
cyclic_stretch increase
H2O2 cyclic_stretch no effect upon treatment-induced increase
low_glucose increase
glucose increase
cyclic_stretch low_glucose augment treatment-induced increase
cyclic_stretch glucose augment treatment-induced increase
AG1478 decrease
cyclic_stretch AG1478 inhibit treatment-induced decrease

Y185-p - ERK2 (mouse)
Modsite: HtGFLtEyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'renal, podocyte'
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H2O2 increase
cyclic_stretch increase
H2O2 cyclic_stretch no effect upon treatment-induced increase
low_glucose increase
glucose increase
cyclic_stretch low_glucose augment treatment-induced increase
cyclic_stretch glucose augment treatment-induced increase
AG1478 decrease
cyclic_stretch AG1478 inhibit treatment-induced decrease

S9-p - GSK3B (mouse)
Modsite: SGRPRttsFAEsCKP SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S9‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'renal, podocyte'
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H2O2 increase
cyclic_stretch increase
H2O2 cyclic_stretch augment treatment-induced increase
AG1478 no change compared to control
cyclic_stretch AG1478 increase slight increase

T183-p - JNK1 (mouse)
Modsite: AGtsFMMtPyVVtRY SwissProt Entrez-Gene
Orthologous residues
JNK1 (human): T183‑p, JNK1 iso2 (human): T183‑p, JNK1 iso3 (human): T183‑p, JNK1 (mouse): T183‑p, JNK1 (rat): T183‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'renal, podocyte'
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H2O2 increase
cyclic_stretch increase
H2O2 cyclic_stretch augment treatment-induced increase
AG1478 no change compared to control
cyclic_stretch AG1478 increase slight increase
low_glucose increase slight increase
glucose increase slight increase
cyclic_stretch glucose augment treatment-induced increase
cyclic_stretch low_glucose augment treatment-induced increase

Y185-p - JNK1 (mouse)
Modsite: tsFMMtPyVVtRYYR SwissProt Entrez-Gene
Orthologous residues
JNK1 (human): Y185‑p, JNK1 iso2 (human): Y185‑p, JNK1 iso3 (human): Y185‑p, JNK1 (mouse): Y185‑p, JNK1 (rat): Y185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'renal, podocyte'
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H2O2 increase
cyclic_stretch increase
H2O2 cyclic_stretch augment treatment-induced increase
AG1478 no change compared to control
cyclic_stretch AG1478 increase slight increase
low_glucose increase slight increase
glucose increase slight increase
cyclic_stretch glucose augment treatment-induced increase
cyclic_stretch low_glucose augment treatment-induced increase

T180-p - P38A (mouse)
Modsite: RHtDDEMtGyVAtRW SwissProt Entrez-Gene
Orthologous residues
P38A (human): T180‑p, P38A iso2 (human): T180‑p, P38A (mouse): T180‑p, P38A iso3 (mouse): T180‑p, P38A (rat): T180‑p, P38A (salmonid): T181‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'renal, podocyte'
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PGE2 increase
PGE2 PTGER4 (mouse) increase siRNA decreases
glucose increase
PGE2 glucose augment treatment-induced increase
H2O2 increase
cyclic_stretch increase slight increase
H2O2 cyclic_stretch inhibit treatment-induced increase
low_glucose increase
cyclic_stretch glucose augment treatment-induced increase
cyclic_stretch low_glucose augment treatment-induced increase

Y182-p - P38A (mouse)
Modsite: tDDEMtGyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
P38A (human): Y182‑p, P38A iso2 (human): Y182‑p, P38A (mouse): Y182‑p, P38A iso3 (mouse): Y182‑p, P38A (rat): Y182‑p, P38A (salmonid): Y183‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'renal, podocyte'
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PGE2 increase
PGE2 PTGER4 (mouse) increase siRNA decreases
glucose increase
PGE2 glucose augment treatment-induced increase
H2O2 increase
cyclic_stretch increase slight increase
H2O2 cyclic_stretch inhibit treatment-induced increase
low_glucose increase
cyclic_stretch glucose augment treatment-induced increase
cyclic_stretch low_glucose augment treatment-induced increase