Garcia-Haro L, et al. (2010) The PP1-R6 protein phosphatase holoenzyme is involved in the glucose-induced dephosphorylation and inactivation of AMP-activated protein kinase, a key regulator of insulin secretion, in MIN6 beta cells. FASEB J 24, 5080-91 20724523

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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S428-p - LKB1 (human) ▲

Modsite: SSkIRRLsACkQQ__ SwissProt Entrez-Gene Orthologous residues LKB1 (human): S428‑p, LKB1 iso2 (human): ‑, LKB1 (mouse): S431‑p, LKB1 (rat): S431‑p, LKB1 (pig): S339‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  pancreatic cancer, pancreatic carcinoma Relevant cell lines - cell types - tissues:  MIN6 (pancreatic) Cellular systems studied:  cell lines Species studied:  human

T183-p - AMPKA1 (mouse) ▲

Modsite: sDGEFLRtsCGsPNY SwissProt Entrez-Gene Orthologous residues AMPKA1 (human): T183‑p, AMPKA1 iso2 (human): T198‑p, AMPKA1 (mouse): T183‑p, AMPKA1 (rat): T183‑p, AMPKA1 (fruit fly): T184‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  pancreatic cancer, pancreatic carcinoma Relevant cell lines - cell types - tissues:  MIN6 (pancreatic) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme PHOSPHATASE PPP2CA (human) PHOSPHATASE PPP1CA (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes PHOSPHATASE PPP1CB (mouse) phospho-antibody, siRNA inhibition of enzyme in complex with the PPP1R3D (R6) regulatory subunit PHOSPHATASE PPP1CA (mouse) phospho-antibody, siRNA inhibition of enzyme in complex with the PPP1R3D (R6) regulatory subunit Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes glucose GK (mouse) decrease glucose PPP1R3D (mouse) GK (mouse) inhibit treatment-induced decrease glucose PPP1R3E (mouse) GK (mouse) inhibit treatment-induced decrease glucose PPP1R3C (human) GK (mouse) no effect upon treatment-induced decrease fructose no change compared to control galactose no change compared to control 2-deoxyglucose no change compared to control