Curated Information
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Home > Curated Information Page > PubMed Id: 20736160
Ayuso MI, et al. (2010) New hierarchical phosphorylation pathway of the translational repressor eIF4E-binding protein 1 (4E-BP1) in ischemia-reperfusion stress. J Biol Chem 285, 34355-63 20736160
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T36-p - 4E-BP1 (rat)
Modsite: PPGDYsttPGGtLFS SwissProt Entrez-Gene
Orthologous residues
4E‑BP1 (human): T37‑p, 4E‑BP1 (mouse): T36‑p, 4E‑BP1 (rat): T36‑p, 4E‑BP1 (fruit fly): T37‑p, 4E‑BP1 (cow): T37‑p
Characterization
Methods used to characterize site in vivo 2D analysis, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'brain, cerebral cortex', 'brain, hippocampus'
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ischemia/reperfusion increase
ischemia decrease
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  translation, altered
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
EIF4E (rat) Disrupts translation, altered pull-down assay

T45-p - 4E-BP1 (rat)
Modsite: GGtLFSttPGGtRII SwissProt Entrez-Gene
Orthologous residues
4E‑BP1 (human): T46‑p, 4E‑BP1 (mouse): T45‑p, 4E‑BP1 (rat): T45‑p, 4E‑BP1 (fruit fly): T46‑p, 4E‑BP1 (cow): T46‑p
Characterization
Methods used to characterize site in vivo 2D analysis, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'brain, cerebral cortex', 'brain, hippocampus'
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ischemia/reperfusion increase
ischemia decrease
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  translation, altered
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
EIF4E (rat) Disrupts translation, altered pull-down assay

S64-p - 4E-BP1 (rat)
Modsite: FLMECRNsPVAKtPP SwissProt Entrez-Gene
Orthologous residues
4E‑BP1 (human): S65‑p, 4E‑BP1 (mouse): S64‑p, 4E‑BP1 (rat): S64‑p, 4E‑BP1 (fruit fly): S65‑p, 4E‑BP1 (cow): S65‑p
Characterization
Methods used to characterize site in vivo 2D analysis, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'brain, cerebral cortex', 'brain, hippocampus'
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ischemia/reperfusion increase
ischemia decrease

T69-p - 4E-BP1 (rat)
Modsite: RNsPVAKtPPKDLPT SwissProt Entrez-Gene
Orthologous residues
4E‑BP1 (human): T70‑p, 4E‑BP1 (mouse): T69‑p, 4E‑BP1 (rat): T69‑p, 4E‑BP1 (fruit fly): T70‑p, 4E‑BP1 (cow): T70‑p
Characterization
Methods used to characterize site in vivo 2D analysis, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'brain, cerebral cortex', 'brain, hippocampus'
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ischemia no change compared to control
ischemia/reperfusion increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  translation, altered
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
EIF4E (rat) Induces translation, altered pull-down assay

T308-p - Akt1 (rat)
Modsite: KDGATMKtFCGTPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo 2D analysis, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'brain, cerebral cortex', 'brain, hippocampus'
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ischemia no change compared to control
ischemia/reperfusion increase

S473-p - Akt1 (rat)
Modsite: RPHFPQFsYSASGTA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo 2D analysis, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'brain, cerebral cortex', 'brain, hippocampus'
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ischemia decrease
ischemia/reperfusion increase
ischemia decrease

S2448-p - mTOR (rat)
Modsite: RSRTRTDsYSAGQSV SwissProt Entrez-Gene
Orthologous residues
mTOR (human): S2448‑p, mTOR (mouse): S2448‑p, mTOR (rat): S2448‑p
Characterization
Methods used to characterize site in vivo 2D analysis, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'brain, cerebral cortex', 'brain, hippocampus'
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ischemia decrease
ischemia decrease