Curated Information
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Home > Curated Information Page > PubMed Id: 20710027
Chandrakesan P, et al. (2010) Novel changes in NF-{kappa}B activity during progression and regression phases of hyperplasia: role of MEK, ERK, and p38. J Biol Chem 285, 33485-98 20710027
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T180-p - P38A (human)
Modsite: RHtDDEMtGyVAtRW SwissProt Entrez-Gene
Orthologous residues
P38A (human): T180‑p, P38A iso2 (human): T180‑p, P38A (mouse): T180‑p, P38A iso3 (mouse): T180‑p, P38A (rat): T180‑p, P38A (salmonid): T181‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  colon
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  young adult mouse colon cells (YAMC)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
bacterial infection increase C. rodentium infection-induced transmissible murine colonic hyperplasia (TMCH), cellular and nuclear extracts

Y182-p - P38A (human)
Modsite: tDDEMtGyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
P38A (human): Y182‑p, P38A iso2 (human): Y182‑p, P38A (mouse): Y182‑p, P38A iso3 (mouse): Y182‑p, P38A (rat): Y182‑p, P38A (salmonid): Y183‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  colon
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  young adult mouse colon cells (YAMC)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
bacterial infection increase C. rodentium infection-induced transmissible murine colonic hyperplasia (TMCH), cellular and nuclear extracts

T203-p - ERK1 (mouse)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  colon
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  young adult mouse colon cells (YAMC)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
bacterial infection increase C. rodentium infection-induced transmissible murine colonic hyperplasia (TMCH), cellular and nuclear extracts
bacterial infection increase
PD98059 bacterial infection inhibit treatment-induced increase

Y205-p - ERK1 (mouse)
Modsite: HtGFLtEyVAtRWyR SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  colon
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  young adult mouse colon cells (YAMC)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
bacterial infection increase C. rodentium infection-induced transmissible murine colonic hyperplasia (TMCH), cellular and nuclear extracts
bacterial infection increase
PD98059 bacterial infection inhibit treatment-induced increase

T183-p - ERK2 (mouse)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  colon
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  young adult mouse colon cells (YAMC)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
bacterial infection increase C. rodentium infection-induced transmissible murine colonic hyperplasia (TMCH), cellular and nuclear extracts
bacterial infection increase
PD98059 bacterial infection inhibit treatment-induced increase

Y185-p - ERK2 (mouse)
Modsite: HtGFLtEyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  colon
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  young adult mouse colon cells (YAMC)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
bacterial infection increase C. rodentium infection-induced transmissible murine colonic hyperplasia (TMCH), cellular and nuclear extracts
bacterial infection increase
PD98059 bacterial infection inhibit treatment-induced increase

S32-p - IkB-alpha (mouse)
Modsite: LVDDRHDsGLDsMKD SwissProt Entrez-Gene
Orthologous residues
IkB‑alpha (human): S32‑p, IkB‑alpha (mouse): S32‑p, IkB‑alpha (rat): S32‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  colon
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  young adult mouse colon cells (YAMC)

S36-p - IkB-alpha (mouse)
Modsite: RHDsGLDsMKDEEYE SwissProt Entrez-Gene
Orthologous residues
IkB‑alpha (human): S36‑p, IkB‑alpha (mouse): S36‑p, IkB‑alpha (rat): S36‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  colon
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  young adult mouse colon cells (YAMC)

S176-p - IKKA (mouse)
Modsite: AKDVDQGsLCTsFVG SwissProt Entrez-Gene
Orthologous residues
IKKA (human): S176‑p, IKKA (mouse): S176‑p, IKKA (rat): S176‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  colon
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  young adult mouse colon cells (YAMC)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
bacterial infection increase
PD98059 bacterial infection inhibit treatment-induced increase

S180-p - IKKA (mouse)
Modsite: DQGsLCTsFVGTLQY SwissProt Entrez-Gene
Orthologous residues
IKKA (human): S180‑p, IKKA (mouse): S180‑p, IKKA (rat): S180‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  colon
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  young adult mouse colon cells (YAMC)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
bacterial infection increase
PD98059 bacterial infection inhibit treatment-induced increase

S177-p - IKKB (mouse)
Modsite: AKELDQGsLCTsFVG SwissProt Entrez-Gene
Orthologous residues
IKKB (human): S177‑p, IKKB (mouse): S177‑p, IKKB (rat): S177‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  colon
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  young adult mouse colon cells (YAMC)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
bacterial infection increase
PD98059 bacterial infection inhibit treatment-induced increase

S181-p - IKKB (mouse)
Modsite: DQGsLCTsFVGTLQy SwissProt Entrez-Gene
Orthologous residues
IKKB (human): S181‑p, IKKB (mouse): S181‑p, IKKB (rat): S181‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  colon
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  young adult mouse colon cells (YAMC)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
bacterial infection increase
PD98059 bacterial infection inhibit treatment-induced increase

S218-p - MEK1 (mouse)
Modsite: VSGQLIDsMANsFVG SwissProt Entrez-Gene
Orthologous residues
MEK1 (human): S218‑p, MEK1 iso2 (human): S192‑p, MEK1 (mouse): S218‑p, MEK1 (rat): S218‑p, MEK1 (rabbit): S218‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  colon
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  young adult mouse colon cells (YAMC)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
bacterial infection increase C. rodentium infection-induced transmissible murine colonic hyperplasia (TMCH), cellular and nuclear extracts

S222-p - MEK1 (mouse)
Modsite: LIDsMANsFVGTRSY SwissProt Entrez-Gene
Orthologous residues
MEK1 (human): S222‑p, MEK1 iso2 (human): S196‑p, MEK1 (mouse): S222‑p, MEK1 (rat): S222‑p, MEK1 (rabbit): S222‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  colon
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  young adult mouse colon cells (YAMC)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
bacterial infection increase C. rodentium infection-induced transmissible murine colonic hyperplasia (TMCH), cellular and nuclear extracts

S222-p - MEK2 (mouse)
Modsite: VsGQLIDsMANsFVG SwissProt Entrez-Gene
Orthologous residues
MEK2 (human): S222‑p, MEK2 (mouse): S222‑p, MEK2 (rat): S222‑p, MEK2 (chicken): S220‑p, MEK2 (cow): S222‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  colon
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  young adult mouse colon cells (YAMC)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
bacterial infection increase C. rodentium infection-induced transmissible murine colonic hyperplasia (TMCH), cellular and nuclear extracts

S226-p - MEK2 (mouse)
Modsite: LIDsMANsFVGTRSY SwissProt Entrez-Gene
Orthologous residues
MEK2 (human): S226‑p, MEK2 (mouse): S226‑p, MEK2 (rat): S226‑p, MEK2 (chicken): S224‑p, MEK2 (cow): S226‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  colon
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  young adult mouse colon cells (YAMC)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
bacterial infection increase C. rodentium infection-induced transmissible murine colonic hyperplasia (TMCH), cellular and nuclear extracts

K310-ac - NFkB-p65 (mouse)
Modsite: KRTYETFksIMKKSP SwissProt Entrez-Gene
Orthologous residues
NFkB‑p65 (human): K310‑ac, NFkB‑p65 (mouse): K310‑ac, NFkB‑p65 (rat): K310‑ac
Characterization
Methods used to characterize site in vivo modification-specific antibody
Relevant cell lines - cell types - tissues:  colon
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  young adult mouse colon cells (YAMC)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
bacterial infection increase C. rodentium infection-induced transmissible murine colonic hyperplasia (TMCH), cellular and nuclear extracts

S534-p - NFkB-p65 (mouse)
Modsite: sGDEDFssIADMDFS SwissProt Entrez-Gene
Orthologous residues
NFkB‑p65 (human): S536‑p, NFkB‑p65 (mouse): S534‑p, NFkB‑p65 (rat): S535‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  colon
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  young adult mouse colon cells (YAMC)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
bacterial infection increase C. rodentium infection-induced transmissible murine colonic hyperplasia (TMCH), cellular and nuclear extracts
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
p90RSK (mouse) Induces co-immunoprecipitation

T348-p - p90RSK (mouse)
Modsite: DTEFTSRtPRDsPGI SwissProt Entrez-Gene
Orthologous residues
p90RSK (human): T359‑p, p90RSK iso2 (human): T368‑p, p90RSK (mouse): T348‑p, p90RSK iso3 (mouse): , p90RSK (rat): T359‑p, p90RSK (chicken): T377‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  colon
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  young adult mouse colon cells (YAMC)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
bacterial infection increase C. rodentium infection-induced transmissible murine colonic hyperplasia (TMCH), cellular and nuclear extracts

S352-p - p90RSK (mouse)
Modsite: TSRtPRDsPGIPPsA SwissProt Entrez-Gene
Orthologous residues
p90RSK (human): S363‑p, p90RSK iso2 (human): S372‑p, p90RSK (mouse): S352‑p, p90RSK iso3 (mouse): , p90RSK (rat): S363‑p, p90RSK (chicken): S381‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  colon
Cellular systems studied:  cell lines
Species studied:  mouse
Comments:  young adult mouse colon cells (YAMC)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
bacterial infection increase C. rodentium infection-induced transmissible murine colonic hyperplasia (TMCH), cellular and nuclear extracts