Curated Information
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Home > Curated Information Page > PubMed Id: 20804212
Tao Z, et al. (2010) Steady-state kinetic and inhibition studies of the mammalian target of rapamycin (mTOR) kinase domain and mTOR complexes. Biochemistry 49, 8488-98 20804212
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T37-p - 4E-BP1 (human)
Modsite: PPGDysttPGGtLFs SwissProt Entrez-Gene
Orthologous residues
4E‑BP1 (human): T37‑p, 4E‑BP1 (mouse): T36‑p, 4E‑BP1 (rat): T36‑p, 4E‑BP1 (fruit fly): T37‑p, 4E‑BP1 (cow): T37‑p

T46-p - 4E-BP1 (human)
Modsite: GGtLFsttPGGtRII SwissProt Entrez-Gene
Orthologous residues
4E‑BP1 (human): T46‑p, 4E‑BP1 (mouse): T45‑p, 4E‑BP1 (rat): T45‑p, 4E‑BP1 (fruit fly): T46‑p, 4E‑BP1 (cow): T46‑p

S473-p - Akt1 (human)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p

S2454-p - mTOR (human)
Modsite: DsysAGQsVEILDGV SwissProt Entrez-Gene
Orthologous residues
mTOR (human): S2454‑p, mTOR (mouse): S2454‑p, mTOR (rat): S2454‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE mTOR (human)

T2473-p - mTOR (human)
Modsite: PAHKKtGttVPEsIH SwissProt Entrez-Gene
Orthologous residues
mTOR (human): T2473‑p, mTOR (mouse): T2473‑p, mTOR (rat): T2473‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE mTOR (human)

T2474-p - mTOR (human)
Modsite: AHKKtGttVPEsIHs SwissProt Entrez-Gene
Orthologous residues
mTOR (human): T2474‑p, mTOR (mouse): T2474‑p, mTOR (rat): T2474‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE mTOR (human)

S2478-p - mTOR (human)
Modsite: tGttVPEsIHsFIGD SwissProt Entrez-Gene
Orthologous residues
mTOR (human): S2478‑p, mTOR (mouse): S2478‑p, mTOR (rat): S2478‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE mTOR (human)
Comments:  S2478 or S2481

S2481-p - mTOR (human)
Modsite: tVPEsIHsFIGDGLV SwissProt Entrez-Gene
Orthologous residues
mTOR (human): S2481‑p, mTOR (mouse): S2481‑p, mTOR (rat): S2481‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mass spectrometry (in vitro)
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE mTOR (human)
Comments:  S2478 or S2481

T412-p - p70S6K (human)
Modsite: NQVFLGFtyVAPsVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p