Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.6.0.1
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 31461649
Chatterjee N, et al. (2019) Synthetic Essentiality of Metabolic Regulator PDHK1 in PTEN-Deficient Cells and Cancers. Cell Rep 28, 2317-2330.e8 31461649
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

S9-p - NKAP (human)
Modsite: APVSGsRsPDREAsG SwissProt Entrez-Gene
Orthologous residues
NKAP (human): S9‑p, NKAP (mouse): S9‑p, NKAP (rat): S9‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  lung cancer, non-small cell lung cancer, melanoma skin cancer
Relevant cell lines - cell types - tissues:  A2058 (keratinocyte), HEK293T (epithelial), NCI-H1650 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
PHOSPHATASE PTEN (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PTEN (human) transfection of wild-type enzyme, genetic knockout/knockin of upstream enzyme, transfection of inactive enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  intracellular localization
Effect of modification (process):  signaling pathway regulation, transcription, induced
Comments:  enhances NF-KB nuclear localization, regulates PTEN/NKAP/NF-KB/PDHK1 signaling

S149-p - NKAP (human)
Modsite: APEVWGLsPkNPEPD SwissProt Entrez-Gene
Orthologous residues
NKAP (human): S149‑p, NKAP (mouse): S147‑p, NKAP (rat): S147‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  lung cancer, non-small cell lung cancer, melanoma skin cancer
Relevant cell lines - cell types - tissues:  A2058 (keratinocyte), HEK293T (epithelial), NCI-H1650 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
PHOSPHATASE PTEN (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PTEN (human) transfection of wild-type enzyme, genetic knockout/knockin of upstream enzyme, transfection of inactive enzyme, co-immunoprecipitation
Downstream Regulation
Effect of modification (function):  intracellular localization
Effect of modification (process):  signaling pathway regulation, transcription, induced
Comments:  enhances NF-KB nuclear localization, regulates PTEN/NKAP/NF-KB/PDHK1 signaling

Y138-p - PTEN (human)
Modsite: TGVMICAyLLHRGKF SwissProt Entrez-Gene
Orthologous residues
PTEN (human): Y138‑p, PTEN iso2 (human): Y311‑p, PTEN (mouse): Y138‑p, PTEN (rat): Y138‑p
Downstream Regulation
Effect of modification (function):  phosphorylation
Effect of modification (process):  signaling pathway regulation, transcription, inhibited
Comments:  induces NKAP dephosphorylation and suppresses RELA phosphorylation, regulates PTEN/NKAP/NF-KB/PDHK1 signaling

S72-p - Rab7 (human)
Modsite: AGQERFQsLGVAFYR SwissProt Entrez-Gene
Orthologous residues
Rab7 (human): S72‑p, Rab7 (mouse): S72‑p, Rab7 (rat): S72‑p
Characterization
Enzymes shown to modify site in vitro
Type Enzyme
PHOSPHATASE PTEN (human)