Takahasi K, et al. (2010) Ser386 phosphorylation of transcription factor IRF-3 induces dimerization and association with CBP/p300 without overall conformational change. Genes Cells 15, 901-10 20604809

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S386-p - IRF3 (human) ▲

Modsite: ARVGGAssLENtVDL SwissProt Entrez-Gene Orthologous residues IRF3 (human): S386‑p, IRF3 (mouse): S379‑p, IRF3 (rat): S381‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, western blotting Disease tissue studied:  fibrosarcoma of soft tissue Relevant cell lines - cell types - tissues:  L929 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Enzymes shown to modify site in vitro:  Type Enzyme KINASE IKKE (human) Downstream Regulation Effect of modification (function):  molecular association, regulation, protein conformation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays p300 (human) Induces co-immunoprecipitation

S398-p - IRF3 (human) ▲
Modsite: VDLHIsNsHPLsLts SwissProt Entrez-Gene Orthologous residues IRF3 (human): S398‑p, IRF3 (mouse): S390‑p, IRF3 (rat): S392‑p Characterization Methods used to characterize site in vivo:  mass spectrometry (in vitro) Enzymes shown to modify site in vitro:  Type Enzyme KINASE IKKE (human)

S402-p - IRF3 (human) ▲

Modsite: IsNsHPLsLtsDQYK SwissProt Entrez-Gene Orthologous residues IRF3 (human): S402‑p, IRF3 (mouse): S394‑p, IRF3 (rat): S396‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, western blotting Disease tissue studied:  fibrosarcoma of soft tissue Relevant cell lines - cell types - tissues:  L929 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Enzymes shown to modify site in vitro:  Type Enzyme KINASE IKKE (human)