Curated Information
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Home > Curated Information Page > PubMed Id: 20606012
Zheng Y, et al. (2010) Protein tyrosine kinase 6 directly phosphorylates AKT and promotes AKT activation in response to epidermal growth factor. Mol Cell Biol 30, 4280-92 20606012
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T308-p - Akt1 (human)
Modsite: kDGAtMKtFCGtPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  prostate cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), BPH-1 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF Brk (human) increase

Y315-p - Akt1 (human)
Modsite: tFCGtPEyLAPEVLE SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): Y315‑p, Akt1 iso2 (human): Y253‑p, Akt1 (mouse): Y315‑p, Akt1 (rat): Y315‑p, Akt1 (fruit fly): Y430‑p, Akt1 (cow): Y315‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro), mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Brk (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Brk (human) transfection of constitutively active enzyme, co-immunoprecipitation, mutation in upstream enzyme recognition motif
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced
Comments:  Brk induces Akt activation by phosphorylation on Y315,Y326.

Y326-p - Akt1 (human)
Modsite: EVLEDNDyGRAVDWW SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): Y326‑p, Akt1 iso2 (human): Y264‑p, Akt1 (mouse): Y326‑p, Akt1 (rat): Y326‑p, Akt1 (fruit fly): Y441‑p, Akt1 (cow): Y326‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro), mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Brk (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Brk (human) transfection of constitutively active enzyme, co-immunoprecipitation, mutation in upstream enzyme recognition motif
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced
Comments:  Brk induces Akt activation by phosphorylation on Y315,Y326.

S473-p - Akt1 (human)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  prostate cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), BPH-1 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF Brk (human) increase

T308-p - Akt1 (mouse)
Modsite: KDGAtMKtFCGtPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  SYF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF Brk (human) increase

Y315-p - Akt1 (mouse)
Modsite: tFCGtPEyLAPEVLE SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): Y315‑p, Akt1 iso2 (human): Y253‑p, Akt1 (mouse): Y315‑p, Akt1 (rat): Y315‑p, Akt1 (fruit fly): Y430‑p, Akt1 (cow): Y315‑p
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced
Comments:  Brk induces Akt activation by phosphorylation on Y315,Y326.

Y326-p - Akt1 (mouse)
Modsite: EVLEDNDyGRAVDWW SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): Y326‑p, Akt1 iso2 (human): Y264‑p, Akt1 (mouse): Y326‑p, Akt1 (rat): Y326‑p, Akt1 (fruit fly): Y441‑p, Akt1 (cow): Y326‑p
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced
Comments:  Brk induces Akt activation by phosphorylation on Y315,Y326.

S473-p - Akt1 (mouse)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  SYF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF Brk (human) increase

T203-p - ERK1 (mouse)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  SYF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

Y205-p - ERK1 (mouse)
Modsite: HtGFLtEyVAtRWyR SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  SYF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse

T183-p - ERK2 (mouse)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  SYF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase
EGF Brk (mouse) no effect upon treatment-induced increase

Y185-p - ERK2 (mouse)
Modsite: HtGFLtEyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  SYF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase
EGF Brk (mouse) no effect upon treatment-induced increase