Drobic B, et al. (2010) Promoter chromatin remodeling of immediate-early genes is mediated through H3 phosphorylation at either serine 28 or 10 by the MSK1 multi-protein complex. Nucleic Acids Res 38, 3196-208 20129940

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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K9-ac - H3 (mouse) ▲

Modsite: RtkQTARkstGGkAP SwissProt Entrez-Gene Orthologous residues H3 (human): K9‑ac, H3 (mouse): K9‑ac, H3 iso2 (mouse): K9‑ac, H3 iso3 (mouse): K9‑ac, H3 (rat): K9‑ac, H3 iso3 (rat): K9‑ac, H3 (pig): K9‑ac, H3 (chicken): K9‑ac, H3 (cow): K9‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, modification-specific antibody Relevant cell lines - cell types - tissues:  C3H10T1/2 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes phorbol_ester increase Downstream Regulation Effect of modification (function):  molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces co-immunoprecipitation

S10-p - H3 (mouse) ▲

Modsite: tkQTARkstGGkAPr SwissProt Entrez-Gene Orthologous residues H3 (human): S10‑p, H3 (mouse): S10‑p, H3 iso2 (mouse): S10‑p, H3 iso3 (mouse): S10‑p, H3 (rat): S10‑p, H3 iso3 (rat): S10‑p, H3 (pig): S10‑p, H3 (chicken): S10‑p, H3 (cow): S10‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  C3H10T1/2 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE MSK1 (human) co-immunoprecipitation, siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme, phospho-antibody Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes phorbol_ester increase Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  chromatin organization, altered, transcription, altered Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces co-immunoprecipitation 14-3-3 epsilon (mouse) Induces co-immunoprecipitation 14-3-3 zeta (mouse) Induces co-immunoprecipitation

K14-ac - H3 (mouse) ▲

Modsite: ARkstGGkAPrkQLA SwissProt Entrez-Gene Orthologous residues H3 (human): K14‑ac, H3 (mouse): K14‑ac, H3 iso2 (mouse): K14‑ac, H3 iso3 (mouse): K14‑ac, H3 (rat): K14‑ac, H3 iso3 (rat): K14‑ac, H3 (pig): K14‑ac, H3 (chicken): K14‑ac, H3 (cow): K14‑ac Characterization Methods used to characterize site in vivo:  immunoprecipitation, modification-specific antibody Relevant cell lines - cell types - tissues:  C3H10T1/2 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes phorbol_ester increase Downstream Regulation Effect of modification (function):  molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces co-immunoprecipitation

S28-p - H3 (mouse) ▲

Modsite: ATkAArksAPATGGV SwissProt Entrez-Gene Orthologous residues H3 (human): S28‑p, H3 (mouse): S28‑p, H3 iso2 (mouse): S28‑p, H3 iso3 (mouse): S28‑p, H3 (rat): S28‑p, H3 iso3 (rat): S28‑p, H3 (pig): S28‑p, H3 (chicken): S28‑p, H3 (cow): S28‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  C3H10T1/2 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE MSK1 (human) co-immunoprecipitation, siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme, phospho-antibody Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes phorbol_ester increase Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  chromatin organization, altered, transcription, altered Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays 14-3-3 epsilon (mouse) Induces co-immunoprecipitation Induces co-immunoprecipitation 14-3-3 zeta (mouse) Induces co-immunoprecipitation