Curated Information
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Home > Curated Information Page > PubMed Id: 20580720
Lee G, et al. (2010) Phosphoinositide 3-kinase signaling mediates beta-catenin activation in intestinal epithelial stem and progenitor cells in colitis. Gastroenterology 139, 869-81, 881.e1-9 20580720
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S552-p - CTNNB1 (human)
Modsite: QDtQRRtsMGGtQQQ SwissProt Entrez-Gene
Orthologous residues
CTNNB1 (human): S552‑p, CTNNB1 (mouse): S552‑p, CTNNB1 (rat): S552‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  colon
Cellular systems studied:  tissue
Species studied:  human
Associated Diseases
Diseases Alterations Comments
colonic inflamation increased chronic ulcerative colitis (CUC), ulcerative colitis associated dysplasia (UCD), colitis-associated cancer (CAC)

T308-p - Akt1 (mouse)
Modsite: KDGAtMKtFCGtPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  epithelial [PIK3R1 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-CD3 PIK3R1 (mouse) increase PIK3R1 knockout inhibits increase
piroxicam increase piroxicam induced colitis in IL-10 -/- mice
LY294002 piroxicam inhibit treatment-induced increase
Associated Diseases
Diseases Alterations Comments
colonic inflamation increased chronic ulcerative colitis (CUC), ulcerative colitis associated dysplasia (UCD), colitis-associated cancer (CAC)

S33-p - CTNNB1 (mouse)
Modsite: QQQsYLDsGIHsGAT SwissProt Entrez-Gene
Orthologous residues
CTNNB1 (human): S33‑p, CTNNB1 (mouse): S33‑p, CTNNB1 (rat): S33‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  epithelial [PIK3R1 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PIK3R1 (mouse) decrease PIK3R1 knockout increases (cytosolic fraction)

S37-p - CTNNB1 (mouse)
Modsite: YLDsGIHsGATtTAP SwissProt Entrez-Gene
Orthologous residues
CTNNB1 (human): S37‑p, CTNNB1 (mouse): S37‑p, CTNNB1 (rat): S37‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  epithelial [PIK3R1 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PIK3R1 (mouse) decrease PIK3R1 knockout increases (cytosolic fraction)

T41-p - CTNNB1 (mouse)
Modsite: GIHsGATtTAPsLSG SwissProt Entrez-Gene
Orthologous residues
CTNNB1 (human): T41‑p, CTNNB1 (mouse): T41‑p, CTNNB1 (rat): T41‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  epithelial [PIK3R1 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PIK3R1 (mouse) decrease PIK3R1 knockout increases (cytosolic fraction)

S552-p - CTNNB1 (mouse)
Modsite: QDTQRRtsMGGtQQQ SwissProt Entrez-Gene
Orthologous residues
CTNNB1 (human): S552‑p, CTNNB1 (mouse): S552‑p, CTNNB1 (rat): S552‑p
Characterization
Methods used to characterize site in vivo microscopy-colocalization with upstream kinase, phospho-antibody
Relevant cell lines - cell types - tissues:  epithelial [PIK3R1 (mouse), homozygous knockout]
Cellular systems studied:  tissue
Species studied:  mouse
Comments:  intestinal epithelial cells
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PIK3R1 (mouse) increase PIK3R1 knockout decreases (nuclear fraction)
anti-CD3 PIK3R1 (mouse) increase PIK3R1 knockout inhibits increase
piroxicam increase piroxicam induced colitis in IL-10 -/- mice
LY294002 piroxicam inhibit treatment-induced increase