Lee G, et al. (2010) Phosphoinositide 3-kinase signaling mediates beta-catenin activation in intestinal epithelial stem and progenitor cells in colitis. Gastroenterology 139, 869-81, 881.e1-9 20580720

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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S552-p - CTNNB1 (human) ▲

Modsite: QDtQRRtsMGGtQQQ SwissProt Entrez-Gene Orthologous residues CTNNB1 (human): S552‑p, CTNNB1 (mouse): S552‑p, CTNNB1 (rat): S552‑p, CTNNB1 (rabbit): S552‑p Characterization Methods used to characterize site in vivo:  phospho-antibody Relevant cell lines - cell types - tissues:  colon Cellular systems studied:  tissue Species studied:  human Associated Diseases Diseases Alterations Comments colonic inflamation increased chronic ulcerative colitis (CUC), ulcerative colitis associated dysplasia (UCD), colitis-associated cancer (CAC)

T308-p - Akt1 (mouse) ▲

Modsite: KDGAtMKtFCGtPEy SwissProt Entrez-Gene Orthologous residues Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p Characterization Methods used to characterize site in vivo:  phospho-antibody Relevant cell lines - cell types - tissues:  epithelial [PIK3R1 (mouse), homozygous knockout] Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes antibody PIK3R1 (mouse) increase PIK3R1 knockout inhibits increase; anti-CD3 piroxicam increase piroxicam induced colitis in IL-10 -/- mice LY294002 piroxicam inhibit treatment-induced increase Associated Diseases Diseases Alterations Comments colonic inflamation increased chronic ulcerative colitis (CUC), ulcerative colitis associated dysplasia (UCD), colitis-associated cancer (CAC)

S33-p - CTNNB1 (mouse) ▲

Modsite: QQQsYLDsGIHsGAT SwissProt Entrez-Gene Orthologous residues CTNNB1 (human): S33‑p, CTNNB1 (mouse): S33‑p, CTNNB1 (rat): S33‑p, CTNNB1 (rabbit): S33‑p Characterization Methods used to characterize site in vivo:  phospho-antibody Relevant cell lines - cell types - tissues:  epithelial [PIK3R1 (mouse), homozygous knockout] Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes PIK3R1 (mouse) decrease PIK3R1 knockout increases (cytosolic fraction)

S37-p - CTNNB1 (mouse) ▲

Modsite: YLDsGIHsGATtTAP SwissProt Entrez-Gene Orthologous residues CTNNB1 (human): S37‑p, CTNNB1 (mouse): S37‑p, CTNNB1 (rat): S37‑p, CTNNB1 (rabbit): S37‑p Characterization Methods used to characterize site in vivo:  phospho-antibody Relevant cell lines - cell types - tissues:  epithelial [PIK3R1 (mouse), homozygous knockout] Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes PIK3R1 (mouse) decrease PIK3R1 knockout increases (cytosolic fraction)

T41-p - CTNNB1 (mouse) ▲

Modsite: GIHsGATtTAPsLsG SwissProt Entrez-Gene Orthologous residues CTNNB1 (human): T41‑p, CTNNB1 (mouse): T41‑p, CTNNB1 (rat): T41‑p, CTNNB1 (rabbit): T41‑p Characterization Methods used to characterize site in vivo:  phospho-antibody Relevant cell lines - cell types - tissues:  epithelial [PIK3R1 (mouse), homozygous knockout] Cellular systems studied:  tissue Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes PIK3R1 (mouse) decrease PIK3R1 knockout increases (cytosolic fraction)

S552-p - CTNNB1 (mouse) ▲

Modsite: QDTQRRtsMGGtQQQ SwissProt Entrez-Gene Orthologous residues CTNNB1 (human): S552‑p, CTNNB1 (mouse): S552‑p, CTNNB1 (rat): S552‑p, CTNNB1 (rabbit): S552‑p Characterization Methods used to characterize site in vivo:  microscopy-colocalization with upstream kinase, phospho-antibody Relevant cell lines - cell types - tissues:  epithelial [PIK3R1 (mouse), homozygous knockout] Cellular systems studied:  tissue Species studied:  mouse Comments:  intestinal epithelial cells Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes PIK3R1 (mouse) increase PIK3R1 knockout decreases (nuclear fraction) antibody PIK3R1 (mouse) increase PIK3R1 knockout inhibits increase; anti-CD3 piroxicam increase piroxicam induced colitis in IL-10 -/- mice LY294002 piroxicam inhibit treatment-induced increase