Curated Information
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Home > Curated Information Page > PubMed Id: 20570725
Moïse N, et al. (2010) Characterization of a novel MK3 splice variant from murine ventricular myocardium. Cell Signal 22, 1502-12 20570725
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T222-p - MAPKAPK2 (human)
Modsite: tSHNsLttPCytPYY SwissProt Entrez-Gene
Orthologous residues
MAPKAPK2 (human): T222‑p, MAPKAPK2 iso2 (human): T222‑p, MAPKAPK2 (mouse): T208‑p, MAPKAPK2 (rat): T208‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sorbitol increase

T180-p - P38A (human)
Modsite: RHtDDEMtGyVAtRW SwissProt Entrez-Gene
Orthologous residues
P38A (human): T180‑p, P38A iso2 (human): T180‑p, P38A (mouse): T180‑p, P38A iso3 (mouse): T180‑p, P38A (rat): T180‑p, P38A (salmonid): T181‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sorbitol increase
SB203580 sorbitol no effect upon treatment-induced increase
SB203580 no change compared to control

Y182-p - P38A (human)
Modsite: tDDEMtGyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
P38A (human): Y182‑p, P38A iso2 (human): Y182‑p, P38A (mouse): Y182‑p, P38A iso3 (mouse): Y182‑p, P38A (rat): Y182‑p, P38A (salmonid): Y183‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
sorbitol increase
SB203580 sorbitol no effect upon treatment-induced increase
SB203580 no change compared to control

T203-p - MAPKAPK3 (mouse)
Modsite: TTQNALQtPCYTPYY SwissProt Entrez-Gene
Orthologous residues
MAPKAPK3 (human): T201‑p, MAPKAPK3 (mouse): T203‑p, MAPKAPK3 (rat): T203‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

S253-p - MAPKAPK3 (mouse)
Modsite: SNTGQAIsPGMKRRI SwissProt Entrez-Gene
Orthologous residues
MAPKAPK3 (human): S251‑p, MAPKAPK3 (mouse): S253‑p, MAPKAPK3 (rat): S253‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

T315-p - MAPKAPK3 (mouse)
Modsite: QSMVVPQtPLYTARV SwissProt Entrez-Gene
Orthologous residues
MAPKAPK3 (human): T313‑p, MAPKAPK3 (mouse): T315‑p, MAPKAPK3 (rat): T315‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human