Curated Information
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Home > Curated Information Page > PubMed Id: 20538596
Scholz R, et al. (2010) Autoactivation of transforming growth factor beta-activated kinase 1 is a sequential bimolecular process. J Biol Chem 285, 25753-66 20538596
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T172-p - AMPKA2 (human)
Modsite: sDGEFLRtsCGsPNy SwissProt Entrez-Gene
Orthologous residues
AMPKA2 (human): T172‑p, AMPKA2 (mouse): T172‑p, AMPKA2 (rat): T172‑p, AMPKA2 (pig): T172‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TAK1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE TAK1 (human) transfection of inactive enzyme, transfection of wild-type enzyme, activation of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H2O2 increase
TAB1 (human) increase activates TAK1

T178-p - TAK1 (human)
Modsite: LKICDFGtACDIQtH SwissProt Entrez-Gene
Orthologous residues
TAK1 (human): T178‑p, TAK1 (mouse): T178‑p, TAK1 iso3 (mouse): T178‑p, TAK1 (rat): T178‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  E.coli (bacterial)
Cellular systems studied:  cell lines
Species studied:  bacteria
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE TAK1 (human) transfection of inactive enzyme
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, phosphorylation
Comments:  facilitates autophosphorylation of T184 and T187

T184-p - TAK1 (human)
Modsite: GtACDIQtHMtNNKG SwissProt Entrez-Gene
Orthologous residues
TAK1 (human): T184‑p, TAK1 (mouse): T184‑p, TAK1 iso3 (mouse): T184‑p, TAK1 (rat): T184‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  E.coli (bacterial)
Cellular systems studied:  cell lines
Species studied:  bacteria
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE TAK1 (human) transfection of inactive enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TAB1 (human) increase
TAB2 (human) TAB1 (human) inhibit treatment-induced increase
TAB2 (human) no change compared to control
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced
Comments:  promotes TAK1 activity , but not mandatory

T187-p - TAK1 (human)
Modsite: CDIQtHMtNNKGsAA SwissProt Entrez-Gene
Orthologous residues
TAK1 (human): T187‑p, TAK1 (mouse): T187‑p, TAK1 iso3 (mouse): T187‑p, TAK1 (rat): T187‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  E.coli (bacterial)
Cellular systems studied:  cell lines
Species studied:  bacteria
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE TAK1 (human) transfection of inactive enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TAB1 (human) increase
TAB2 (human) TAB1 (human) inhibit treatment-induced increase
TAB2 (human) no change compared to control
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S192-p - TAK1 (human)
Modsite: HMtNNKGsAAWMAPE SwissProt Entrez-Gene
Orthologous residues
TAK1 (human): S192‑p, TAK1 (mouse): S192‑p, TAK1 iso3 (mouse): S192‑p, TAK1 (rat): S192‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  E.coli (bacterial)
Cellular systems studied:  cell lines
Species studied:  bacteria
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE TAK1 (human) transfection of inactive enzyme
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced