Curated Information
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Home > Curated Information Page > PubMed Id: 20571032
Weinman EJ, et al. (2010) Cooperativity between the phosphorylation of Thr95 and Ser77 of NHERF-1 in the hormonal regulation of renal phosphate transport. J Biol Chem 285, 25134-8 20571032
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S77-p - NHERF (human)
Modsite: ETHQQVVsRIRAALN SwissProt Entrez-Gene
Orthologous residues
NHERF (human): S77‑p, NHERF (mouse): S77‑p, NHERF (rat): S77‑p, NHERF (rabbit): S77‑p, NHERF (dog): S77‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site
Relevant cell lines - cell types - tissues:  OK (renal)
Cellular systems studied:  primary cultured cells
Species studied:  opossum
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCA (human)
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SLC34A1 (human) Disrupts plasmon resonance

T95-p - NHERF (human)
Modsite: LLVVDPEtDEQLQkL SwissProt Entrez-Gene
Orthologous residues
NHERF (human): T95‑p, NHERF (mouse): T95‑p, NHERF (rat): T95‑p, NHERF (rabbit): T95‑p, NHERF (dog): A95‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site
Relevant cell lines - cell types - tissues:  OK (renal)
Cellular systems studied:  primary cultured cells
Species studied:  opossum
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PTH increase
dopamine increase
Downstream Regulation
Effect of modification (function):  phosphorylation
Comments:  facilitates phosphorylation of S77