Curated Information
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Home > Curated Information Page > PubMed Id: 20028775
Collins M, et al. (2010) The T cell receptor-mediated phosphorylation of Pyk2 tyrosines 402 and 580 occurs via a distinct mechanism than other receptor systems. J Leukoc Biol 87, 691-701 20028775
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S473-p - Akt1 (human)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  lymphoma, T cell lymphoma
Relevant cell lines - cell types - tissues:  HUT-78 (T lymphocyte), Jurkat (T lymphocyte), P116 (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-CD3 increase
wortmannin anti-CD3 inhibit treatment-induced increase

Y220-p - LAT (human)
Modsite: sLDGsREyVNVsQEL SwissProt Entrez-Gene
Orthologous residues
LAT (human): Y220‑p, LAT iso2 (human): Y191‑p, LAT iso3 (human): Y227‑p, LAT iso4 (human): Y190‑p, LAT (mouse): Y195‑p, LAT (rat): Y195‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  lymphoma, T cell lymphoma
Relevant cell lines - cell types - tissues:  HUT-78 (T lymphocyte), Jurkat (T lymphocyte), P116 (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
damnacanthal increase
ZAP70 (human) increase P116 ZAP70 as compared to parental P116

Y402-p - Pyk2 (human)
Modsite: CsIEsDIyAEIPDEt SwissProt Entrez-Gene
Orthologous residues
Pyk2 (human): Y402‑p, Pyk2 iso2 (human): Y402‑p, Pyk2 (mouse): Y402‑p, Pyk2 (rat): Y402‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  lymphoma, T cell lymphoma
Relevant cell lines - cell types - tissues:  HUT-78 (T lymphocyte), Jurkat (T lymphocyte), P116 (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-CD3 increase
PP2 anti-CD3 inhibit treatment-induced increase
damnacanthal increase
ZAP70 (human) no effect upon treatment-induced increase P116 ZAP70 as compared to parental P116
wortmannin anti-CD3 no effect upon treatment-induced increase
latrunculin_B anti-CD3 no effect upon treatment-induced increase
EGTA anti-CD3 no effect upon treatment-induced increase
BAPTA-AM anti-CD3 no effect upon treatment-induced increase
EGTA, BAPTA-AM anti-CD3 no effect upon treatment-induced increase
Fyn (human) decrease Fyn siRNA increases

Y580-p - Pyk2 (human)
Modsite: yIEDEDyykAsVTRL SwissProt Entrez-Gene
Orthologous residues
Pyk2 (human): Y580‑p, Pyk2 iso2 (human): Y580‑p, Pyk2 (mouse): Y580‑p, Pyk2 (rat): Y580‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  lymphoma, T cell lymphoma
Relevant cell lines - cell types - tissues:  HUT-78 (T lymphocyte), Jurkat (T lymphocyte), P116 (T lymphocyte)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
anti-CD3 increase
PP2 anti-CD3 inhibit treatment-induced increase
damnacanthal increase
ZAP70 (human) no effect upon treatment-induced increase P116 ZAP70 as compared to parental P116
wortmannin anti-CD3 no effect upon treatment-induced increase
latrunculin_B anti-CD3 no effect upon treatment-induced increase
EGTA anti-CD3 no effect upon treatment-induced increase
BAPTA-AM anti-CD3 no effect upon treatment-induced increase
EGTA, BAPTA-AM anti-CD3 no effect upon treatment-induced increase
Fyn (human) increase Fyn siRNA decreases