Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 17928297
Morimoto H, et al. (2007) Activation of a C-terminal transcriptional activation domain of ERK5 by autophosphorylation. J Biol Chem 282, 35449-56 17928297
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

S421-p - ERK5 (mouse)
Modsite: CPDVEMPsPWAPSGD SwissProt Entrez-Gene
Orthologous residues
ERK5 (human): S421‑p, ERK5 iso2 (human): S282‑p, ERK5 (mouse): S421‑p, ERK5 (rat): S421‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey

S433-p - ERK5 (mouse)
Modsite: SGDCAMEsPPPALPP SwissProt Entrez-Gene
Orthologous residues
ERK5 (human): S433‑p, ERK5 iso2 (human): S294‑p, ERK5 (mouse): S433‑p, ERK5 (rat): S433‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey

S496-p - ERK5 (mouse)
Modsite: SRLRDGPsAPLEAPE SwissProt Entrez-Gene
Orthologous residues
ERK5 (human): S496‑p, ERK5 iso2 (human): S357‑p, ERK5 (mouse): S496‑p, ERK5 (rat): S496‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey

S721-p - ERK5 (mouse)
Modsite: DPLPPVFsGtPKGSG SwissProt Entrez-Gene
Orthologous residues
ERK5 (human): S731‑p, ERK5 iso2 (human): S592‑p, ERK5 (mouse): S721‑p, ERK5 (rat): S721‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey

T723-p - ERK5 (mouse)
Modsite: LPPVFsGtPKGSGAG SwissProt Entrez-Gene
Orthologous residues
ERK5 (human): T733‑p, ERK5 iso2 (human): T594‑p, ERK5 (mouse): T723‑p, ERK5 (rat): T723‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK5 (mouse)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK5 (mouse) transfection of wild-type enzyme

S760-p - ERK5 (mouse)
Modsite: PQDGQADsASLsAsL SwissProt Entrez-Gene
Orthologous residues
ERK5 (human): S770‑p, ERK5 iso2 (human): S631‑p, ERK5 (mouse): S760‑p, ERK5 (rat): S760‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK5 (mouse)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK5 (mouse) transfection of wild-type enzyme

S764-p - ERK5 (mouse)
Modsite: QADsASLsAsLLADW SwissProt Entrez-Gene
Orthologous residues
ERK5 (human): S774‑p, ERK5 iso2 (human): S635‑p, ERK5 (mouse): S764‑p, ERK5 (rat): S764‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK5 (mouse)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK5 (mouse) transfection of wild-type enzyme

S766-p - ERK5 (mouse)
Modsite: DsASLsAsLLADWLE SwissProt Entrez-Gene
Orthologous residues
ERK5 (human): S776‑p, ERK5 iso2 (human): S637‑p, ERK5 (mouse): S766‑p, ERK5 (rat): S766‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK5 (mouse)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK5 (mouse) transfection of wild-type enzyme