Koren I, Reem E, Kimchi A (2010) DAP1, a novel substrate of mTOR, negatively regulates autophagy. Curr Biol 20, 1093-8 20537536

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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T37-p - 4E-BP1 (human) ▲
Modsite: PPGDysttPGGtLFs SwissProt Entrez-Gene Orthologous residues 4E‑BP1 (human): T37‑p, 4E‑BP1 (mouse): T36‑p, 4E‑BP1 (rat): T36‑p, 4E‑BP1 (fruit fly): T37‑p, 4E‑BP1 (cow): T37‑p Characterization Enzymes shown to modify site in vitro:  Type Enzyme KINASE mTOR (human)

T46-p - 4E-BP1 (human) ▲
Modsite: GGtLFsttPGGtRII SwissProt Entrez-Gene Orthologous residues 4E‑BP1 (human): T46‑p, 4E‑BP1 (mouse): T45‑p, 4E‑BP1 (rat): T45‑p, 4E‑BP1 (fruit fly): T46‑p, 4E‑BP1 (cow): T46‑p Characterization Enzymes shown to modify site in vitro:  Type Enzyme KINASE mTOR (human)

S3-p - DAP (human) ▲

Modsite: _____MssPPEGkLE SwissProt Entrez-Gene Orthologous residues DAP (human): S3‑p, DAP (mouse): S3‑p, DAP (rat): S3‑p Characterization Methods used to characterize site in vivo:  electrophoretic mobility shift, immunoprecipitation, mass spectrometry, mutation of modification site Relevant cell lines - cell types - tissues:  293 (epithelial), HEK293T (epithelial), HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes amino_acid_starvation decrease refeeding increase Torin1 decrease rapamycin decrease Downstream Regulation Effect of modification (function):  activity, inhibited Effect of modification (process):  autophagy, altered Comments:  dephosphorylated form of DAP acts as an active suppressor of autophagy

S51-p - DAP (human) ▲

Modsite: DQEWEsPsPPkPtVF SwissProt Entrez-Gene Orthologous residues DAP (human): S51‑p, DAP (mouse): S51‑p, DAP (rat): S51‑p Characterization Methods used to characterize site in vivo:  electrophoretic mobility shift, immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), HEK293T (epithelial), HeLa (cervical) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE mTOR (human) Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes amino_acid_starvation decrease refeeding increase Torin1 decrease rapamycin decrease Downstream Regulation Effect of modification (function):  activity, inhibited Effect of modification (process):  autophagy, altered Comments:  dephosphorylated form of DAP acts as an active suppressor of autophagy