Gehret AU, Hinkle PM (2010) Importance of regions outside the cytoplasmic tail of G-protein-coupled receptors for phosphorylation and dephosphorylation. Biochem J 428, 235-45 20345371

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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S262-p - ADRB2 (human) ▲

Modsite: GHGLRRssKFcLKEH SwissProt Entrez-Gene Orthologous residues ADRB2 (human): S262‑p, ADRB2 (mouse): S262‑p, ADRB2 (rat): S262‑p, ADRB2 (hamster): S262‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Comments:  TRHR-ADRB2 chimearic protein Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes isoproterenol increase H-89 isoproterenol inhibit treatment-induced increase

S355-p - ADRB2 (human) ▲

Modsite: KAyGNGyssNGNtGE SwissProt Entrez-Gene Orthologous residues ADRB2 (human): S355‑p, ADRB2 (mouse): S355‑p, ADRB2 (rat): S355‑p, ADRB2 (hamster): S355‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Comments:  TRHR-ADRB2 chimearic protein Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes isoproterenol increase TRH increase Downstream Regulation Effect of modification (function):  receptor desensitization, altered, receptor internalization, altered

S356-p - ADRB2 (human) ▲

Modsite: AyGNGyssNGNtGEQ SwissProt Entrez-Gene Orthologous residues ADRB2 (human): S356‑p, ADRB2 (mouse): S356‑p, ADRB2 (rat): S356‑p, ADRB2 (hamster): S356‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Comments:  TRHR-ADRB2 chimearic protein Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes isoproterenol increase TRH increase Downstream Regulation Effect of modification (function):  receptor desensitization, altered, receptor internalization, altered

S355-p - TRHR (rat) ▲

Modsite: YSVALNYsVIKEsDR SwissProt Entrez-Gene Orthologous residues TRHR (human): S355‑p, TRHR (mouse): S355‑p, TRHR (rat): S355‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Comments:  ADRB2-TRHR chimearic protein Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes TRH increase Downstream Regulation Effect of modification (function):  receptor internalization, altered

S360-p - TRHR (rat) ▲

Modsite: NYsVIKEsDRFstEL SwissProt Entrez-Gene Orthologous residues TRHR (human): S360‑p, TRHR (mouse): S360‑p, TRHR (rat): S360‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Comments:  ADRB2-TRHR chimearic protein Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes TRH increase Downstream Regulation Effect of modification (function):  receptor internalization, altered

S364-p - TRHR (rat) ▲

Modsite: IKEsDRFstELDDIt SwissProt Entrez-Gene Orthologous residues TRHR (human): S364‑p, TRHR (mouse): S364‑p, TRHR (rat): S364‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Comments:  ADRB2-TRHR chimearic protein Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes TRH increase Downstream Regulation Effect of modification (function):  receptor internalization, altered

T365-p - TRHR (rat) ▲

Modsite: KEsDRFstELDDItV SwissProt Entrez-Gene Orthologous residues TRHR (human): T365‑p, TRHR (mouse): T365‑p, TRHR (rat): T365‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Comments:  ADRB2-TRHR chimearic protein Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes isoproterenol increase TRH increase Downstream Regulation Effect of modification (function):  receptor internalization, altered