Curated Information
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Home > Curated Information Page > PubMed Id: 20303298
Nava P, et al. (2010) Interferon-gamma regulates intestinal epithelial homeostasis through converging beta-catenin signaling pathways. Immunity 32, 392-402 20303298
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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T308-p - Akt1 (human)
Modsite: kDGAtMKtFCGtPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  T84 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IFN-gamma increase 5 hr
TNF increase
TNF IFN-gamma augment treatment-induced increase
IFN-gamma increase 72 hr
TNF increase slight increase
TNF IFN-gamma no effect upon treatment-induced increase

S552-p - CTNNB1 (human)
Modsite: QDtQRRtsMGGtQQQ SwissProt Entrez-Gene
Orthologous residues
CTNNB1 (human): S552‑p, CTNNB1 (mouse): S552‑p, CTNNB1 (rat): S552‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  T84 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IFN-gamma increase 5 hr
TNF increase
TNF IFN-gamma augment treatment-induced increase

S1490-p - LRP6 (human)
Modsite: AILNPPPsPAtERsH SwissProt Entrez-Gene
Orthologous residues
LRP6 (human): S1490‑p, LRP6 (mouse): S1490‑p, LRP6 (rat):
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  T84 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IFN-gamma increase 5 hr treatment
TNF increase
TNF IFN-gamma no effect upon treatment-induced increase
IFN-gamma decrease 72 hr
TNF increase
TNF IFN-gamma no effect upon treatment-induced decrease

T308-p - Akt1 (mouse)
Modsite: KDGAtMKtFCGtPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  epithelial
Cellular systems studied:  primary cells
Species studied:  mouse
Comments:  intestinal epithelial cells
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
inflammation increase dextran sulfate sodium
IL-6 no change compared to control
IL-22 no change compared to control
inflammation increase dextran sulfate sodium
triciribine no change compared to control
triciribine inflammation inhibit treatment-induced increase

S552-p - CTNNB1 (mouse)
Modsite: QDTQRRtsMGGtQQQ SwissProt Entrez-Gene
Orthologous residues
CTNNB1 (human): S552‑p, CTNNB1 (mouse): S552‑p, CTNNB1 (rat): S552‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  epithelial
Cellular systems studied:  primary cells
Species studied:  mouse
Comments:  intestinal epithelial cells
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
inflammation increase dextran sulfate sodium
IL-6 no change compared to control
IL-22 no change compared to control
inflammation increase dextran sulfate sodium
triciribine no change compared to control
triciribine inflammation inhibit treatment-induced increase
IFNG (mouse) increase dextran sulfate sodium, IFNG -/- decreases

S9-p - GSK3B (mouse)
Modsite: SGRPRttsFAEsCKP SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S9‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  epithelial
Cellular systems studied:  primary cells
Species studied:  mouse
Comments:  intestinal epithelial cells
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
inflammation increase dextran sulfate sodium
IL-6 no change compared to control
IL-22 no change compared to control

S1490-p - LRP6 (mouse)
Modsite: AILNPPPsPAtERSH SwissProt Entrez-Gene
Orthologous residues
LRP6 (human): S1490‑p, LRP6 (mouse): S1490‑p, LRP6 (rat):
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  epithelial
Cellular systems studied:  primary cells
Species studied:  mouse
Comments:  intestinal epithelial cells