Curated Information
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Home > Curated Information Page > PubMed Id: 31096047
Meng Z, et al. Kinase activity of casein kinase 1 delta (CK1δ) is modulated by protein kinase C α (PKCα) by site-specific phosphorylation within the kinase domain of CK1δ. Biochim Biophys Acta Proteins Proteom 1867, 710-721 31096047
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T161-p - CK1D (human)
Modsite: KKYRDARtHQHIPYR SwissProt Entrez-Gene
Orthologous residues
CK1D (human): T161‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry (in vitro), mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCA (human)

T174-p - CK1D (human)
Modsite: YRENkNLtGtARYAs SwissProt Entrez-Gene
Orthologous residues
CK1D (human): T174‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry (in vitro), mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCA (human)

T176-p - CK1D (human)
Modsite: ENkNLtGtARYAsIN SwissProt Entrez-Gene
Orthologous residues
CK1D (human): T176‑p, CK1D iso2 (human): T176‑p, CK1D (mouse): T176‑p, CK1D iso2 (mouse): T176‑p, CK1D (rat): T176‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCA (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKCA (human) pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Go_6983 decrease
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S181-p - CK1D (human)
Modsite: tGtARYAsINTHLGI SwissProt Entrez-Gene
Orthologous residues
CK1D (human): S181‑p, CK1D iso2 (human): S181‑p, CK1D (mouse): S181‑p, CK1D iso2 (mouse): S181‑p, CK1D (rat): S181‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry (in vitro), mutation of modification site, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCA (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKCA (human) pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Go_6983 decrease

S191-p - CK1D (human)
Modsite: THLGIEQsRRDDLES SwissProt Entrez-Gene
Orthologous residues
CK1D (human): S191‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry (in vitro), mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCA (human)

S298-p - CK1D (human)
Modsite: NMLKFGAsRAADDAE SwissProt Entrez-Gene
Orthologous residues
CK1D (human): S298‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry (in vitro), mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCA (human)