Curated Information
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Home > Curated Information Page > PubMed Id: 20456499
Schröter J, et al. (2010) Homologous desensitization of guanylyl cyclase A, the receptor for atrial natriuretic peptide, is associated with a complex phosphorylation pattern. FEBS J 277, 2440-53 20456499
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S515-p - ANPA (rat)
Modsite: WEDLQPSsLERHLRS SwissProt Entrez-Gene
Orthologous residues
ANPA (human): S519‑p, ANPA (mouse): S515‑p, ANPA (rat): S515‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ANP increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, inhibited, protein conformation, receptor desensitization, altered

S525-p - ANPA (rat)
Modsite: RHLRSAGsRLtLsGR SwissProt Entrez-Gene
Orthologous residues
ANPA (human): S529‑p, ANPA (mouse): S525‑p, ANPA (rat): S525‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ANP increase

T528-p - ANPA (rat)
Modsite: RSAGsRLtLsGRGsN SwissProt Entrez-Gene
Orthologous residues
ANPA (human): T532‑p, ANPA (mouse): T528‑p, ANPA (rat): T528‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ANP increase

S530-p - ANPA (rat)
Modsite: AGsRLtLsGRGsNYG SwissProt Entrez-Gene
Orthologous residues
ANPA (human): S534‑p, ANPA (mouse): S530‑p, ANPA (rat): S530‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ANP increase

S534-p - ANPA (rat)
Modsite: LtLsGRGsNYGsLLt SwissProt Entrez-Gene
Orthologous residues
ANPA (human): S538‑p, ANPA (mouse): S534‑p, ANPA (rat): S534‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

S538-p - ANPA (rat)
Modsite: GRGsNYGsLLtTEGQ SwissProt Entrez-Gene
Orthologous residues
ANPA (human): S542‑p, ANPA (mouse): S538‑p, ANPA (rat): S538‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

T541-p - ANPA (rat)
Modsite: sNYGsLLtTEGQFQV SwissProt Entrez-Gene
Orthologous residues
ANPA (human): T545‑p, ANPA (mouse): T541‑p, ANPA (rat): T541‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human