Curated Information
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Home > Curated Information Page > PubMed Id: 20400510
Deguchi A, et al. (2010) LKB1 suppresses p21-activated kinase-1 (PAK1) by phosphorylation of Thr109 in the p21-binding domain. J Biol Chem 285, 18283-90 20400510
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T109-p - PAK1 (human)
Modsite: QWARLLQtsNITKsE SwissProt Entrez-Gene
Orthologous residues
PAK1 (human): T109‑p, PAK1 (mouse): T109‑p, PAK1 (rat): T109‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HCT116 (intestinal), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE LKB1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE LKB1 (mouse) genetic knockout/knockin of upstream enzyme
Downstream Regulation
Effect of modification (function):  enzymatic activity, inhibited
Comments:  in vitro

T109-p - PAK1 (mouse)
Modsite: QWARLLQtSNITKsE SwissProt Entrez-Gene
Orthologous residues
PAK1 (human): T109‑p, PAK1 (mouse): T109‑p, PAK1 (rat): T109‑p
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE LKB1 (mouse) genetic knockout/knockin of upstream enzyme
Downstream Regulation
Effect of modification (function):  enzymatic activity, inhibited
Comments:  in vitro

S144-p - PAK1 (mouse)
Modsite: SNSKKYMsFTDKSAE SwissProt Entrez-Gene
Orthologous residues
PAK1 (human): S144‑p, PAK1 (mouse): S144‑p, PAK1 (rat): S144‑p

S199-p - PAK1 (mouse)
Modsite: PRPEHTKsVYTRsVI SwissProt Entrez-Gene
Orthologous residues
PAK1 (human): S199‑p, PAK1 (mouse): S199‑p, PAK1 (rat): S198‑p

S204-p - PAK1 (mouse)
Modsite: TKsVYTRsVIEPLPV SwissProt Entrez-Gene
Orthologous residues
PAK1 (human): S204‑p, PAK1 (mouse): S204‑p, PAK1 (rat): S203‑p