Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus®
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 20138985
Nascimento EB, et al. (2010) Phosphorylation of PRAS40 on Thr246 by PKB/AKT facilitates efficient phosphorylation of Ser183 by mTORC1. Cell Signal 22, 961-7 20138985
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

S183-p - PRAS40 (human)
Modsite: PTQQYAKsLPVsVPV SwissProt Entrez-Gene
Orthologous residues
PRAS40 (human): S183‑p, PRAS40 iso2 (human): S53‑p, PRAS40 iso3 (human): S203‑p, PRAS40 (mouse): S184‑p, PRAS40 (rat): S184‑p, PRAS40 (cow): S183‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3 (fibroblast), L6 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse, rat
Comments:  phosphorylation of T246 required for S183 phosphorylation
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase

T246-p - PRAS40 (human)
Modsite: LPRPRLNtsDFQkLK SwissProt Entrez-Gene
Orthologous residues
PRAS40 (human): T246‑p, PRAS40 iso2 (human): T116‑p, PRAS40 iso3 (human): T266‑p, PRAS40 (mouse): T247‑p, PRAS40 (rat): T247‑p, PRAS40 (cow): T246‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3 (fibroblast), L6 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse, rat
Comments:  phosphorylation of T246 required for S183 phosphorylation
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase

T308-p - Akt1 (mouse)
Modsite: KDGAtMKtFCGtPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3 (fibroblast), L6 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
amino_acids no change compared to control A14 fibroblasts
insulin insulin increase
insulin amino_acids increase
rapamycin amino_acids no change compared to control
rapamycin insulin no effect upon treatment-induced increase
rapamycin amino_acids, insulin no effect upon treatment-induced increase
wortmannin amino_acids no change compared to control
wortmannin amino_acids, insulin inhibit treatment-induced increase
insulin increase
palmitate insulin inhibit treatment-induced increase

S473-p - Akt1 (mouse)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3 (fibroblast), L6 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
amino_acids no change compared to control A14 fibroblasts
insulin insulin increase
insulin amino_acids increase
rapamycin amino_acids no change compared to control
rapamycin insulin no effect upon treatment-induced increase
rapamycin amino_acids, insulin no effect upon treatment-induced increase
wortmannin amino_acids no change compared to control
wortmannin amino_acids, insulin inhibit treatment-induced increase
insulin increase
palmitate insulin inhibit treatment-induced increase

S2481-p - mTOR (mouse)
Modsite: tVPEsIHsFIGDGLV SwissProt Entrez-Gene
Orthologous residues
mTOR (human): S2481‑p, mTOR (mouse): S2481‑p, mTOR (rat): S2481‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3 (fibroblast), L6 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
amino_acid_starvation increase slight increase, A14 fibroblasts and L6 myotubes
insulin amino_acid_starvation augment treatment-induced increase increase
insulin increase slight iincrease
rapamycin no change compared to control
rapamycin amino_acid_starvation, insulin inhibit treatment-induced increase
rapamycin insulin no effect upon treatment-induced increase no effect on slight increase
wortmannin amino_acid_starvation no effect upon treatment-induced increase no effect on slight increase
wortmannin amino_acid_starvation, insulin inhibit treatment-induced increase

T412-p - p70S6K (mouse)
Modsite: NQVFLGFtYVAPSVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3 (fibroblast), L6 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
amino_acid_starvation increase slight increase, A14 fibroblasts and L6 myotubes
insulin amino_acid_starvation augment treatment-induced increase increase
insulin increase slight iincrease
rapamycin no change compared to control
rapamycin amino_acid_starvation, insulin inhibit treatment-induced increase
rapamycin insulin no effect upon treatment-induced increase no effect on slight increase
wortmannin amino_acid_starvation no effect upon treatment-induced increase no effect on slight increase
wortmannin amino_acid_starvation, insulin inhibit treatment-induced increase

S184-p - PRAS40 (mouse)
Modsite: PTQQYAKsLPVSVPV SwissProt Entrez-Gene
Orthologous residues
PRAS40 (human): S183‑p, PRAS40 iso2 (human): S53‑p, PRAS40 iso3 (human): S203‑p, PRAS40 (mouse): S184‑p, PRAS40 (rat): S184‑p, PRAS40 (cow): S183‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3 (fibroblast), L6 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
amino_acids no change compared to control A14 fibroblasts
insulin insulin increase
insulin amino_acids increase
rapamycin amino_acids no change compared to control
rapamycin insulin no effect upon treatment-induced increase
rapamycin amino_acids, insulin no effect upon treatment-induced increase
wortmannin amino_acids no change compared to control
wortmannin amino_acids, insulin inhibit treatment-induced increase
amino_acid_starvation increase slight increase, A14 fibroblasts
insulin increase slight increase
rapamycin amino_acid_starvation no effect upon treatment-induced increase
rapamycin insulin inhibit treatment-induced increase slight inhibition
rapamycin amino_acid_starvation, insulin inhibit treatment-induced increase slight inhibition
wortmannin amino_acid_starvation inhibit treatment-induced increase slight inhibition
wortmannin amino_acid_starvation, insulin inhibit treatment-induced increase
insulin increase
palmitate insulin inhibit treatment-induced increase

T247-p - PRAS40 (mouse)
Modsite: LPRPRLNtsDFQKLK SwissProt Entrez-Gene
Orthologous residues
PRAS40 (human): T246‑p, PRAS40 iso2 (human): T116‑p, PRAS40 iso3 (human): T266‑p, PRAS40 (mouse): T247‑p, PRAS40 (rat): T247‑p, PRAS40 (cow): T246‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3 (fibroblast), L6 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
palmitate insulin inhibit treatment-induced increase
Downstream Regulation
Effect of modification (function):  phosphorylation
Comments:  required for phosphorylation of PRAS40 S184

T308-p - Akt1 (rat)
Modsite: KDGATMKtFCGTPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3 (fibroblast), L6 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
amino_acid_starvation no change compared to control L6 myotubes
insulin increase slight increase
insulin amino_acid_starvation increase
rapamycin amino_acid_starvation no change compared to control
rapamycin amino_acid_starvation, insulin no effect upon treatment-induced increase
rapamycin insulin no effect upon treatment-induced increase
wortmannin amino_acid_starvation no change compared to control
wortmannin amino_acid_starvation, insulin inhibit treatment-induced increase

S473-p - Akt1 (rat)
Modsite: RPHFPQFsYSASGTA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3 (fibroblast), L6 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
amino_acid_starvation no change compared to control L6 myotubes
insulin increase slight increase
insulin amino_acid_starvation increase
rapamycin amino_acid_starvation no change compared to control
rapamycin amino_acid_starvation, insulin no effect upon treatment-induced increase
rapamycin insulin no effect upon treatment-induced increase
wortmannin amino_acid_starvation no change compared to control
wortmannin amino_acid_starvation, insulin inhibit treatment-induced increase

S2481-p - mTOR (rat)
Modsite: TVPEsIHsFIGDGLV SwissProt Entrez-Gene
Orthologous residues
mTOR (human): S2481‑p, mTOR (mouse): S2481‑p, mTOR (rat): S2481‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3 (fibroblast), L6 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse, rat

T412-p - p70S6K (rat)
Modsite: NQVFLGFtYVAPSVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3 (fibroblast), L6 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
palmitate no change compared to control slight inhibition at high concetration

S184-p - PRAS40 (rat)
Modsite: PTQQYAKsLPVSVPV SwissProt Entrez-Gene
Orthologous residues
PRAS40 (human): S183‑p, PRAS40 iso2 (human): S53‑p, PRAS40 iso3 (human): S203‑p, PRAS40 (mouse): S184‑p, PRAS40 (rat): S184‑p, PRAS40 (cow): S183‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3 (fibroblast), L6 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
amino_acid_starvation increase L6 myotubes
insulin no change compared to control
insulin amino_acid_starvation augment treatment-induced increase
rapamycin no change compared to control
rapamycin amino_acid_starvation, insulin no effect upon treatment-induced increase
rapamycin insulin no change compared to control
wortmannin amino_acid_starvation no effect upon treatment-induced increase
wortmannin amino_acid_starvation, insulin inhibit treatment-induced increase
low-fat diet no change compared to control skeletal muscle
insulin low-fat diet increase
high-fat diet increase slight increase
insulin high-fat diet no effect upon treatment-induced increase
low-fat diet no change compared to control heart muscle
insulin low-fat diet increase
high-fat diet no change compared to control
insulin high-fat diet no change compared to control

T247-p - PRAS40 (rat)
Modsite: LPRPRLNtSDFQKLK SwissProt Entrez-Gene
Orthologous residues
PRAS40 (human): T246‑p, PRAS40 iso2 (human): T116‑p, PRAS40 iso3 (human): T266‑p, PRAS40 (mouse): T247‑p, PRAS40 (rat): T247‑p, PRAS40 (cow): T246‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3 (fibroblast), L6 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
amino_acid_starvation increase L6 myotubes
insulin increase
insulin amino_acid_starvation augment treatment-induced increase
rapamycin amino_acid_starvation inhibit treatment-induced increase
rapamycin amino_acid_starvation, insulin no effect upon treatment-induced increase
rapamycin insulin no effect upon treatment-induced increase
wortmannin amino_acid_starvation inhibit treatment-induced increase
wortmannin amino_acid_starvation, insulin inhibit treatment-induced increase
low-fat diet no change compared to control heart muscle
insulin low-fat diet increase
high-fat diet no change compared to control
insulin high-fat diet no change compared to control
low-fat diet no change compared to control skeletal muscle
insulin low-fat diet increase
high-fat diet no change compared to control
insulin high-fat diet no change compared to control