Curated Information
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Home > Curated Information Page > PubMed Id: 20185632
Lai YC, Zarrinpashneh E, Jensen J (2010) Additive effect of contraction and insulin on glucose uptake and glycogen synthase in muscle with different glycogen contents. J Appl Physiol 108, 1106-15 20185632
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S218-p - ACC2 (rat)
Modsite: PTMRPSMsGLHLVKR SwissProt Entrez-Gene
Orthologous residues
ACC2 (human): S222‑p, ACC2 (mouse): S212‑p, ACC2 (rat): S218‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  rat
Comments:  epitrochlearis muscle
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
muscle contraction increase
fasting muscle contraction no effect upon treatment-induced increase
refeeding muscle contraction no effect upon treatment-induced increase
insulin muscle contraction no effect upon treatment-induced increase
insulin no change compared to control

T308-p - Akt1 (rat)
Modsite: KDGATMKtFCGTPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  rat
Comments:  epitrochlearis muscle
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
fasting insulin augment treatment-induced increase
refeeding insulin inhibit treatment-induced increase
muscle contraction insulin no effect upon treatment-induced increase
muscle contraction no change compared to control

S473-p - Akt1 (rat)
Modsite: RPHFPQFsYSASGTA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  rat
Comments:  epitrochlearis muscle
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
fasting insulin augment treatment-induced increase
refeeding insulin inhibit treatment-induced increase
muscle contraction insulin no effect upon treatment-induced increase
muscle contraction no change compared to control

T183-p - AMPKA1 (rat)
Modsite: SDGEFLRtsCGSPNY SwissProt Entrez-Gene
Orthologous residues
AMPKA1 (human): T183‑p, AMPKA1 iso2 (human): T198‑p, AMPKA1 (mouse): T183‑p, AMPKA1 (rat): T183‑p, AMPKA1 (fruit fly): T184‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  rat
Comments:  epitrochlearis muscle
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
muscle contraction increase
fasting muscle contraction augment treatment-induced increase
refeeding muscle contraction inhibit treatment-induced increase
insulin muscle contraction no effect upon treatment-induced increase
insulin no change compared to control

S9-p - GSK3B (rat)
Modsite: SGRPRTTsFAESCKP SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S9‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  rat
Comments:  epitrochlearis muscle
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
muscle contraction increase
muscle contraction insulin no effect upon treatment-induced increase
fasting muscle contraction inhibit treatment-induced increase

S8-p - GYS1 (rat)
Modsite: MPLSRSLsMSsLPGL SwissProt Entrez-Gene
Orthologous residues
GYS1 (human): S8‑p, GYS1 iso2 (human): S8‑p, GYS1 (mouse): S8‑p, GYS1 (rat): S8‑p, GYS1 (rabbit): S8‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  rat
Comments:  epitrochlearis muscle
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fasting decrease
refeeding increase
muscle contraction no change compared to control
insulin no change compared to control
fasting decrease
refeeding increase slight increase
muscle contraction increase
fasting muscle contraction inhibit treatment-induced increase
insulin muscle contraction no effect upon treatment-induced increase
insulin no change compared to control

S11-p - GYS1 (rat)
Modsite: SRSLsMSsLPGLEDW SwissProt Entrez-Gene
Orthologous residues
GYS1 (human): S11‑p, GYS1 iso2 (human): S11‑p, GYS1 (mouse): S11‑p, GYS1 (rat): S11‑p, GYS1 (rabbit): S11‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  rat
Comments:  epitrochlearis muscle
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fasting decrease
refeeding increase
muscle contraction no change compared to control
insulin no change compared to control

S641-p - GYS1 (rat)
Modsite: YRYPRPAsVPPsPSL SwissProt Entrez-Gene
Orthologous residues
GYS1 (human): S641‑p, GYS1 iso2 (human): S577‑p, GYS1 (mouse): S641‑p, GYS1 (rat): S641‑p, GYS1 (rabbit): S641‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  rat
Comments:  epitrochlearis muscle
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fasting decrease
refeeding decrease
muscle contraction decrease
insulin decrease
insulin muscle contraction augment treatment-induced decrease

S645-p - GYS1 (rat)
Modsite: RPAsVPPsPSLsRHs SwissProt Entrez-Gene
Orthologous residues
GYS1 (human): S645‑p, GYS1 iso2 (human): S581‑p, GYS1 (mouse): S645‑p, GYS1 (rat): S645‑p, GYS1 (rabbit): S645‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  rat
Comments:  epitrochlearis muscle
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fasting decrease
refeeding no change compared to control
muscle contraction decrease
insulin decrease
insulin muscle contraction augment treatment-induced decrease
fasting decrease
refeeding decrease
muscle contraction decrease
insulin decrease
insulin muscle contraction augment treatment-induced decrease

S649-p - GYS1 (rat)
Modsite: VPPsPSLsRHssPHQ SwissProt Entrez-Gene
Orthologous residues
GYS1 (human): S649‑p, GYS1 iso2 (human): S585‑p, GYS1 (mouse): S649‑p, GYS1 (rat): S649‑p, GYS1 (rabbit): S649‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  rat
Comments:  epitrochlearis muscle
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fasting decrease
refeeding no change compared to control
muscle contraction decrease
insulin decrease
insulin muscle contraction augment treatment-induced decrease

S653-p - GYS1 (rat)
Modsite: PSLsRHssPHQsEDE SwissProt Entrez-Gene
Orthologous residues
GYS1 (human): S653‑p, GYS1 iso2 (human): S589‑p, GYS1 (mouse): S653‑p, GYS1 (rat): S653‑p, GYS1 (rabbit): S653‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  rat
Comments:  epitrochlearis muscle
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fasting decrease
refeeding no change compared to control
muscle contraction decrease
insulin decrease
insulin muscle contraction augment treatment-induced decrease

S657-p - GYS1 (rat)
Modsite: RHssPHQsEDEEEPR SwissProt Entrez-Gene
Orthologous residues
GYS1 (human): S657‑p, GYS1 iso2 (human): S593‑p, GYS1 (mouse): S657‑p, GYS1 (rat): S657‑p, GYS1 (rabbit): S657‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal'
Cellular systems studied:  tissue
Species studied:  rat
Comments:  epitrochlearis muscle
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
fasting decrease
refeeding no change compared to control
muscle contraction decrease
insulin decrease
insulin muscle contraction augment treatment-induced decrease