Parker SS, et al. (2019) Insulin Induces Microtubule Stabilization and Regulates the Microtubule Plus-End Tracking Protein Network in Adipocytes. Mol Cell Proteomics 31018989

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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S300-p - CENTG3 (mouse) ▲

Modsite: LAIGPCKsLPNSPSH SwissProt Entrez-Gene Orthologous residues CENTG3 (human): S265‑p, CENTG3 iso2 (human): S266‑p, CENTG3 iso3 (human): S265‑p, CENTG3 iso4 (human): S301‑p, CENTG3 (mouse): S300‑p, CENTG3 (rat): ‑ Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes serum_starvation, insulin increase

S478-p - CENTG3 (mouse) ▲

Modsite: PTTAPGtsPRANGLA SwissProt Entrez-Gene Orthologous residues CENTG3 (human): S443‑p, CENTG3 iso2 (human): S444‑p, CENTG3 iso3 (human): ‑, CENTG3 iso4 (human): S479‑p, CENTG3 (mouse): S478‑p, CENTG3 (rat): S107‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes serum_starvation, insulin increase

S1861-p - CKAP5 (mouse) ▲

Modsite: YEYKKKYsDTDIEPF SwissProt Entrez-Gene Orthologous residues CKAP5 (human): S1861‑p, CKAP5 iso3 (human): S1868‑p, CKAP5 (mouse): S1861‑p, CKAP5 (rat): S1861‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes serum_starvation, insulin decrease

S50-p - CYLN2 (mouse) ▲

Modsite: sPLHKQAsGPsSsGA SwissProt Entrez-Gene Orthologous residues CYLN2 (human): S49‑p, CYLN2 iso2 (human): S49‑p, CYLN2 (mouse): S50‑p, CYLN2 (rat): S50‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes serum_starvation, insulin decrease

S55-p - CYLN2 (mouse) ▲

Modsite: QAsGPsSsGAATTVS SwissProt Entrez-Gene Orthologous residues CYLN2 (human): S54‑p, CYLN2 iso2 (human): S54‑p, CYLN2 (mouse): S55‑p, CYLN2 (rat): A55‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes serum_starvation, insulin decrease

S552-p - CYLN2 (mouse) ▲

Modsite: RLRERLLsASKEHQR SwissProt Entrez-Gene Orthologous residues CYLN2 (human): S551‑p, CYLN2 iso2 (human): S516‑p, CYLN2 (mouse): S552‑p, CYLN2 (rat): S552‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes serum_starvation, insulin increase

S800-p - CYLN2 (mouse) ▲

Modsite: NRLQAAEsLCSAQHS SwissProt Entrez-Gene Orthologous residues CYLN2 (human): A799‑p, CYLN2 iso2 (human): A764‑p, CYLN2 (mouse): S800‑p, CYLN2 (rat): S799‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes serum_starvation, insulin increase

S924-p - CYLN2 (mouse) ▲

Modsite: LLEANRHsPGPERDL SwissProt Entrez-Gene Orthologous residues CYLN2 (human): S923‑p, CYLN2 iso2 (human): S888‑p, CYLN2 (mouse): S924‑p, CYLN2 (rat): S923‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes serum_starvation, insulin increase

S155-p - EB1 (mouse) ▲

Modsite: kPkKPLGsSTAAPQR SwissProt Entrez-Gene Orthologous residues EB1 (human): S155‑p, EB1 (mouse): S155‑p, EB1 (rat): S155‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes serum_starvation, insulin increase

S40-p - MARK2 (mouse) ▲

Modsite: NMLRGRNsAtsADEQ SwissProt Entrez-Gene Orthologous residues MARK2 (human): S40‑p, MARK2 iso4 (human): S40‑p, MARK2 iso10 (human): S7‑p, MARK2 iso11 (human): S40‑p, MARK2 iso13 (human): S7‑p, MARK2 iso14 (human): S7‑p, MARK2 iso16 (human): S40‑p, MARK2 (mouse): S40‑p, MARK2 iso3 (mouse): S40‑p, MARK2 (rat): S40‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes serum_starvation, insulin increase

T42-p - MARK2 (mouse) ▲

Modsite: LRGRNsAtsADEQPH SwissProt Entrez-Gene Orthologous residues MARK2 (human): T42‑p, MARK2 iso4 (human): T42‑p, MARK2 iso10 (human): T9‑p, MARK2 iso11 (human): T42‑p, MARK2 iso13 (human): T9‑p, MARK2 iso14 (human): T9‑p, MARK2 iso16 (human): T42‑p, MARK2 (mouse): T42‑p, MARK2 iso3 (mouse): T42‑p, MARK2 (rat): T42‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes serum_starvation, insulin increase

S43-p - MARK2 (mouse) ▲

Modsite: RGRNsAtsADEQPHI SwissProt Entrez-Gene Orthologous residues MARK2 (human): S43‑p, MARK2 iso4 (human): S43‑p, MARK2 iso10 (human): S10‑p, MARK2 iso11 (human): S43‑p, MARK2 iso13 (human): S10‑p, MARK2 iso14 (human): S10‑p, MARK2 iso16 (human): S43‑p, MARK2 (mouse): S43‑p, MARK2 iso3 (mouse): S43‑p, MARK2 (rat): S43‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes serum_starvation, insulin increase

S568-p - MARK2 (mouse) ▲

Modsite: RVPVAsPsAHNISSS SwissProt Entrez-Gene Orthologous residues MARK2 (human): S571‑p, MARK2 iso4 (human): S517‑p, MARK2 iso10 (human): S484‑p, MARK2 iso11 (human): S571‑p, MARK2 iso13 (human): S483‑p, MARK2 iso14 (human): S537‑p, MARK2 iso16 (human): S516‑p, MARK2 (mouse): S568‑p, MARK2 iso3 (mouse): S514‑p, MARK2 (rat): S514‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes serum_starvation, insulin decrease

S194-p - Restin (mouse) ▲

Modsite: LTKtAsEsIsNLsEA SwissProt Entrez-Gene Orthologous residues Restin (human): S195‑p, Restin iso1 (human): S195‑p, Restin iso3 (human): S195‑p, Restin (mouse): S194‑p, Restin (rat): S194‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes serum_starvation, insulin increase

T286-p - Restin (mouse) ▲

Modsite: KIGFPsTtPAKAKAA SwissProt Entrez-Gene Orthologous residues Restin (human): T287‑p, Restin iso1 (human): T287‑p, Restin iso3 (human): T287‑p, Restin (mouse): T286‑p, Restin (rat): T286‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes serum_starvation, insulin increase

S1317-p - Restin (mouse) ▲

Modsite: EDLNSYDsDDQEKQS SwissProt Entrez-Gene Orthologous residues Restin (human): S1364‑p, Restin iso1 (human): S1353‑p, Restin iso3 (human): S1318‑p, Restin (mouse): S1317‑p, Restin (rat): S1246‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes serum_starvation, insulin increase

K40-ac - TUBA1A (mouse) ▲

Modsite: DGQMPSDktIGGGDD SwissProt Entrez-Gene Orthologous residues TUBA1A (human): K40‑ac, TUBA1A (mouse): K40‑ac, TUBA1A (rat): K40‑ac, TUBA1A (cow): K40‑ac Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes serum_starvation, insulin increase SB216763 increase Downstream Regulation Effect of modification (process):  cytoskeletal reorganization Comments:  leads to microtubule stablization