Curated Information
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Home > Curated Information Page > PubMed Id: 12172553
Inoki K, et al. (2002) TSC2 is phosphorylated and inhibited by Akt and suppresses mTOR signalling. Nat Cell Biol 4, 648-57 12172553
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S939-p - TSC2 (rat)
Modsite: KFRARSTsLNERPKS SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): S939‑p, TSC2 iso2 (human): S939‑p, TSC2 iso3 (human): S939‑p, TSC2 iso4 (human): S939‑p, TSC2 (mouse): S939‑p, TSC2 iso6 (mouse): S939‑p, TSC2 (rat): S939‑p, TSC2 iso2 (rat): S939‑p

S1130-p - TSC2 (rat)
Modsite: GARDRVRsMsGGHGL SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): S1130‑p, TSC2 iso2 (human): S1087‑p, TSC2 iso3 (human): S1086‑p, TSC2 iso4 (human): S1130‑p, TSC2 (mouse): S1130‑p, TSC2 iso6 (mouse): S1130‑p, TSC2 (rat): S1130‑p, TSC2 iso2 (rat): S1087‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  293 (epithelial) [TSC2 iso2 (rat)]
Comments:  The authors used rat tuberin isoform 2 (SP# P49816-2) to characterize the phosphorylation sites.
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)

S1132-p - TSC2 (rat)
Modsite: RDRVRsMsGGHGLRV SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): S1132‑p, TSC2 iso2 (human): S1089‑p, TSC2 iso3 (human): S1088‑p, TSC2 iso4 (human): S1132‑p, TSC2 (mouse): S1132‑p, TSC2 iso6 (mouse): S1132‑p, TSC2 (rat): S1132‑p, TSC2 iso2 (rat): S1089‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  293 (epithelial) [TSC2 iso2 (rat)]
Comments:  The authors used rat tuberin isoform 2 (SP# P49816-2) to characterize the phosphorylation sites.
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)

T1466-p - TSC2 (rat)
Modsite: GLRPRGYtISDSAPS SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): T1462‑p, TSC2 iso2 (human): T1419‑p, TSC2 iso3 (human): T1418‑p, TSC2 iso4 (human): T1439‑p, TSC2 (mouse): T1465‑p, TSC2 iso6 (mouse): T1443‑p, TSC2 (rat): T1466‑p, TSC2 iso2 (rat): T1423‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, immunoprecipitation, mutation of modification site, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  293 (epithelial) [TSC2 iso2 (rat)]
Comments:  The authors used rat tuberin isoform 2 (SP# P49816-2) to characterize the phosphorylation sites.
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)

S939-p - TSC2 iso2 (rat)
Modsite: KFRARSTsLNERPKS SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): S939‑p, TSC2 iso2 (human): S939‑p, TSC2 iso3 (human): S939‑p, TSC2 iso4 (human): S939‑p, TSC2 (mouse): S939‑p, TSC2 iso6 (mouse): S939‑p, TSC2 (rat): S939‑p, TSC2 iso2 (rat): S939‑p

S1087-p - TSC2 iso2 (rat)
Modsite: GARDRVRsMsGGHGL SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): S1130‑p, TSC2 iso2 (human): S1087‑p, TSC2 iso3 (human): S1086‑p, TSC2 iso4 (human): S1130‑p, TSC2 (mouse): S1130‑p, TSC2 iso6 (mouse): S1130‑p, TSC2 (rat): S1130‑p, TSC2 iso2 (rat): S1087‑p

S1089-p - TSC2 iso2 (rat)
Modsite: RDRVRsMsGGHGLRV SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): S1132‑p, TSC2 iso2 (human): S1089‑p, TSC2 iso3 (human): S1088‑p, TSC2 iso4 (human): S1132‑p, TSC2 (mouse): S1132‑p, TSC2 iso6 (mouse): S1132‑p, TSC2 (rat): S1132‑p, TSC2 iso2 (rat): S1089‑p

T1423-p - TSC2 iso2 (rat)
Modsite: GLRPRGYtISDSAPS SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): T1462‑p, TSC2 iso2 (human): T1419‑p, TSC2 iso3 (human): T1418‑p, TSC2 iso4 (human): T1439‑p, TSC2 (mouse): T1465‑p, TSC2 iso6 (mouse): T1443‑p, TSC2 (rat): T1466‑p, TSC2 iso2 (rat): T1423‑p