Lee S, et al. (2010) RSK-mediated phosphorylation in the C/EBP{beta} leucine zipper regulates DNA binding, dimerization, and growth arrest activity. Mol Cell Biol 30, 2621-35 20351173

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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S64-p - C/EBP-beta (rat) ▲
Modsite: HERAIDFsPYLEPLA SwissProt Entrez-Gene Orthologous residues C/EBP‑beta (human): S76‑p, C/EBP‑beta (mouse): S64‑p, C/EBP‑beta (rat): S64‑p, C/EBP‑beta (chicken): S65‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  L929 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

S105-p - C/EBP-beta (rat) ▲
Modsite: AKPSKKPsDYGyVsL SwissProt Entrez-Gene Orthologous residues C/EBP‑beta (human): A135‑p, C/EBP‑beta (mouse): A104‑p, C/EBP‑beta (rat): S105‑p, C/EBP‑beta (chicken): P120‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  L929 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

Y109-p - C/EBP-beta (rat) ▲

Modsite: KKPsDYGyVsLGrAG SwissProt Entrez-Gene Orthologous residues C/EBP‑beta (human): Y139‑p, C/EBP‑beta (mouse): Y108‑p, C/EBP‑beta (rat): Y109‑p, C/EBP‑beta (chicken): Y124‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, mutation of modification site Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  cell growth, induced, transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces

S111-p - C/EBP-beta (rat) ▲

Modsite: PsDYGyVsLGrAGAK SwissProt Entrez-Gene Orthologous residues C/EBP‑beta (human): S141‑p, C/EBP‑beta (mouse): S110‑p, C/EBP‑beta (rat): S111‑p, C/EBP‑beta (chicken): S126‑p Characterization Methods used to characterize site in vivo:  mass spectrometry, mutation of modification site Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  cell growth, induced, transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces

R114-m1 - C/EBP-beta (rat) ▲

Modsite: YGyVsLGrAGAKAAP SwissProt Entrez-Gene Orthologous residues C/EBP‑beta (human): R144‑m1, C/EBP‑beta (mouse): R113‑m1, C/EBP‑beta (rat): R114‑m1, C/EBP‑beta (chicken): R129‑m1 Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  cell growth, induced, transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays Induces

S181-p - C/EBP-beta (rat) ▲
Modsite: SSGsLSTsSSSsPPG SwissProt Entrez-Gene Orthologous residues C/EBP‑beta (human): S227‑p, C/EBP‑beta (mouse): S180‑p, C/EBP‑beta (rat): S181‑p, C/EBP‑beta (chicken): S212‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  L929 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

S185-p - C/EBP-beta (rat) ▲
Modsite: LSTsSSSsPPGtPSP SwissProt Entrez-Gene Orthologous residues C/EBP‑beta (human): S231‑p, C/EBP‑beta (mouse): S184‑p, C/EBP‑beta (rat): S185‑p, C/EBP‑beta (chicken): S216‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  L929 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

T189-p - C/EBP-beta (rat) ▲
Modsite: SSSsPPGtPSPADAK SwissProt Entrez-Gene Orthologous residues C/EBP‑beta (human): T235‑p, C/EBP‑beta (mouse): T188‑p, C/EBP‑beta (rat): T189‑p, C/EBP‑beta (chicken): T220‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  L929 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

S240-p - C/EBP-beta (rat) ▲
Modsite: NNIAVRKsRDKAKMR SwissProt Entrez-Gene Orthologous residues C/EBP‑beta (human): S288‑p, C/EBP‑beta (mouse): S239‑p, C/EBP‑beta (rat): S240‑p, C/EBP‑beta (chicken): S271‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  L929 (fibroblast) Cellular systems studied:  cell lines Species studied:  mouse

S273-p - C/EBP-beta (rat) ▲

Modsite: QKKVEQLsRELSTLR SwissProt Entrez-Gene Orthologous residues C/EBP‑beta (human): S321‑p, C/EBP‑beta (mouse): S272‑p, C/EBP‑beta (rat): S273‑p, C/EBP‑beta (chicken): S304‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mass spectrometry, mutation of modification site Relevant cell lines - cell types - tissues:  HEK293T (epithelial), L929 (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme KINASE RSK2 (mouse) KINASE RSK3 (mouse) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE RSK2 (human) transfection of constitutively active enzyme, activation of upstream enzyme, transfection of dominant-negative enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes serum increase Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  cell cycle regulation, transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays C/EBP-beta (rat) Induces co-immunoprecipitation Induces