Fan Y, et al. (2010) Lysine 63-linked polyubiquitination of TAK1 at lysine 158 is required for tumor necrosis factor alpha- and interleukin-1beta-induced IKK/NF-kappaB and JNK/AP-1 activation. J Biol Chem 285, 5347-60 20038579

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K158-ub - TAK1 (human) ▲

Modsite: ALIHRDLkPPNLLLV SwissProt Entrez-Gene Orthologous residues TAK1 (human): K158‑ub, TAK1 iso2 (human): K158‑ub, TAK1 (mouse): K158‑ub, TAK1 iso3 (mouse): K158‑ub, TAK1 (rat): K158‑ub Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial), HeLa (cervical), MEF (fibroblast) Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme UBIQUITIN LIGASE TRAF6 (human) UBIQUITIN LIGASE TRAF2 (human) UBIQUITIN LIGASE UBE2N (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes UBIQUITIN LIGASE TRAF2 (human) transfection of wild-type enzyme UBIQUITIN LIGASE TRAF6 (human) transfection of wild-type enzyme UBIQUITIN LIGASE UBE2N (human) siRNA inhibition of enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes TNF increase IL-1b increase Downstream Regulation Effect of modification (function):  enzymatic activity, induced, molecular association, regulation Effect of modification (process):  signaling pathway regulation, transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays IKKG (human) Induces co-immunoprecipitation, pull-down assay IKKB (human) Induces pull-down assay