Curated Information
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Home > Curated Information Page > PubMed Id: 18923149
Xu Q, et al. (2009) Regulation of kinetochore recruitment of two essential mitotic spindle checkpoint proteins by Mps1 phosphorylation. Mol Biol Cell 20, 10-20 18923149
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T12-p - TTK (human)
Modsite: DLsGRELtIDsIMNk SwissProt Entrez-Gene
Orthologous residues
TTK (human): T12‑p, TTK iso2 (human): T12‑p, TTK (mouse): T12‑p, TTK iso2 (mouse): T12‑p, TTK (rat): T12‑p, TTK (frog): K12‑p
Characterization
Methods used to characterize site in vivo 2D analysis, [32P] bio-synthetic labeling, immunoprecipitation, mass spectrometry (in vitro), mutation of modification site
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), SW480 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TTK (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase
Downstream Regulation
Effect of modification (function):  intracellular localization
Comments:  regulates kinetochore localization of TTK

S15-p - TTK (human)
Modsite: GRELtIDsIMNkVRD SwissProt Entrez-Gene
Orthologous residues
TTK (human): S15‑p, TTK iso2 (human): S15‑p, TTK (mouse): S15‑p, TTK iso2 (mouse): S15‑p, TTK (rat): S15‑p, TTK (frog): S15‑p
Characterization
Methods used to characterize site in vivo 2D analysis, [32P] bio-synthetic labeling, immunoprecipitation, mass spectrometry (in vitro), mutation of modification site
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), SW480 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TTK (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase
Downstream Regulation
Effect of modification (function):  intracellular localization
Comments:  regulates kinetochore localization of TTK

T33-p - TTK (human)
Modsite: kFkNEDLtDELsLNK SwissProt Entrez-Gene
Orthologous residues
TTK (human): T33‑p, TTK iso2 (human): T33‑p, TTK (mouse): T32‑p, TTK iso2 (mouse): T32‑p, TTK (rat): T32‑p, TTK (frog): T34‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro), mutation of modification site
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  SW480 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TTK (human)

S37-p - TTK (human)
Modsite: EDLtDELsLNKISAD SwissProt Entrez-Gene
Orthologous residues
TTK (human): S37‑p, TTK iso2 (human): S37‑p, TTK (mouse): S36‑p, TTK iso2 (mouse): S36‑p, TTK (rat): N36‑p, TTK (frog): T38‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro), mutation of modification site
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  SW480 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TTK (human)

S80-p - TTK (human)
Modsite: EkNSVPLsDALLNkL SwissProt Entrez-Gene
Orthologous residues
TTK (human): S80‑p, TTK iso2 (human): S80‑p, TTK (mouse): N76‑p, TTK iso2 (mouse): N76‑p, TTK (rat): N76‑p, TTK (frog): D81‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro), mutation of modification site
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  SW480 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TTK (human)

T288-p - TTK (human)
Modsite: sPDCDVktDDsVVPC SwissProt Entrez-Gene
Orthologous residues
TTK (human): T288‑p, TTK iso2 (human): T288‑p, TTK (mouse): T284‑p, TTK iso2 (mouse): T284‑p, TTK (rat): T284‑p, TTK (frog): S291‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro), mutation of modification site
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  SW480 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TTK (human)

T360-p - TTK (human)
Modsite: sITLKNktEssLLAk SwissProt Entrez-Gene
Orthologous residues
TTK (human): T360‑p, TTK iso2 (human): T360‑p, TTK (mouse): T380‑p, TTK iso2 (mouse): T354‑p, TTK (rat): T355‑p, TTK (frog): Q388‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro), mutation of modification site
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  SW480 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TTK (human)

S362-p - TTK (human)
Modsite: TLKNktEssLLAkLE SwissProt Entrez-Gene
Orthologous residues
TTK (human): S362‑p, TTK iso2 (human): S362‑p, TTK (mouse): S382‑p, TTK iso2 (mouse): S356‑p, TTK (rat): S357‑p, TTK (frog): N390‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro), mutation of modification site
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  SW480 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TTK (human)

S363-p - TTK (human)
Modsite: LKNktEssLLAkLEE SwissProt Entrez-Gene
Orthologous residues
TTK (human): S363‑p, TTK iso2 (human): S363‑p, TTK (mouse): S383‑p, TTK iso2 (mouse): S357‑p, TTK (rat): S358‑p, TTK (frog): S391‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro), mutation of modification site
Disease tissue studied:  colorectal cancer, colorectal carcinoma
Relevant cell lines - cell types - tissues:  SW480 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TTK (human)

T675-p - TTK (human)
Modsite: ANQMQPDttsVVkDS SwissProt Entrez-Gene
Orthologous residues
TTK (human): T675‑p, TTK iso2 (human): T674‑p, TTK (mouse): T674‑p, TTK iso2 (mouse): T648‑p, TTK (rat): T653‑p, TTK (frog): V696‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TTK (human)

S677-p - TTK (human)
Modsite: QMQPDttsVVkDSQV SwissProt Entrez-Gene
Orthologous residues
TTK (human): S677‑p, TTK iso2 (human): S676‑p, TTK (mouse): S676‑p, TTK iso2 (mouse): S650‑p, TTK (rat): S655‑p, TTK (frog): S698‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TTK (human)

T686-p - TTK (human)
Modsite: VkDSQVGtVNYMPPE SwissProt Entrez-Gene
Orthologous residues
TTK (human): T686‑p, TTK iso2 (human): T685‑p, TTK (mouse): T685‑p, TTK iso2 (mouse): T659‑p, TTK (rat): T664‑p, TTK (frog): T707‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TTK (human)

S821-p - TTK (human)
Modsite: GQLVGLNsPNsILkA SwissProt Entrez-Gene
Orthologous residues
TTK (human): S821‑p, TTK iso2 (human): S820‑p, TTK (mouse): S820‑p, TTK iso2 (mouse): S794‑p, TTK (rat): S799‑p, TTK (frog): S844‑p
Downstream Regulation
Effect of modification (function):  intracellular localization
Comments:  regulates kinetochore localization of TTK