Zhang L, et al. (2017) SUMO-triggered ubiquitination of NR4A1 controls macrophage cell death. Cell Death Differ 24, 1530-1539 28622293

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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K102-sm - Nur77 (human) ▲

Modsite: sPASASFkFEDFQVY SwissProt Entrez-Gene Orthologous residues Nur77 (human): K102‑sm, Nur77 iso2 (human): K115‑sm, Nur77 (mouse): K104‑sm, Nur77 (rat): K101‑sm Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Disease tissue studied:  leukemia, acute myelogenous leukemia Relevant cell lines - cell types - tissues:  HEK293T (epithelial), HeLa (cervical), THP1 (myeloid) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes SUMO LIGASE PIAS3 (human) transfection of inactive enzyme, microscopy-colocalization, transfection of wild-type enzyme, co-immunoprecipitation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes SENP1 (human) decrease phorbol_ester increase Downstream Regulation Effect of modification (function):  protein degradation, ubiquitination Effect of modification (process):  transcription, inhibited Comments:  inhibits TNF-alpha and IL-1beta-induced NF-kB activity

K577-sm - Nur77 (human) ▲

Modsite: LQRIFYLkLEDLVPP SwissProt Entrez-Gene Orthologous residues Nur77 (human): K577‑sm, Nur77 iso2 (human): K590‑sm, Nur77 (mouse): K580‑sm, Nur77 (rat): K576‑sm Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Disease tissue studied:  leukemia, acute myelogenous leukemia Relevant cell lines - cell types - tissues:  HEK293T (epithelial), HeLa (cervical), THP1 (myeloid) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes SUMO LIGASE PIAS3 (human) transfection of inactive enzyme, microscopy-colocalization, transfection of wild-type enzyme, co-immunoprecipitation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes SENP1 (human) decrease phorbol_ester increase Downstream Regulation Effect of modification (function):  protein degradation, ubiquitination Effect of modification (process):  transcription, inhibited Comments:  inhibits TNF-alpha and IL-1beta-induced NF-kB activity