Shor B, et al. (2010) Requirement of the mTOR kinase for the regulation of Maf1 phosphorylation and control of RNA polymerase III-dependent transcription in cancer cells. J Biol Chem 285, 15380-92 20233713

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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S473-p - Akt1 (human) ▲

Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene Orthologous residues Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  bone cancer, osteosarcoma Relevant cell lines - cell types - tissues:  MDA-MB-361 (breast cell), MG63 (bone cell) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes WYE132 decrease temsirolimus decrease temsirolimus decrease

S60-p - MAF1 (human) ▲

Modsite: PHVLEALsPPQtsGL SwissProt Entrez-Gene Orthologous residues MAF1 (human): S60‑p, MAF1 (mouse): S60‑p, MAF1 (rat): S60‑p Characterization Methods used to characterize site in vivo:  electrophoretic mobility shift, mutation of modification site, western blotting Disease tissue studied:  bone cancer, osteosarcoma Relevant cell lines - cell types - tissues:  293 (epithelial), MG63 (bone cell) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes WYE132 decrease temsirolimus decrease mTOR (human) increase Downstream Regulation Effect of modification (process):  transcription, induced

T64-p - MAF1 (human) ▲

Modsite: EALsPPQtsGLsPsR SwissProt Entrez-Gene Orthologous residues MAF1 (human): T64‑p, MAF1 (mouse): T64‑p, MAF1 (rat): T64‑p Characterization Methods used to characterize site in vivo:  electrophoretic mobility shift, mutation of modification site, western blotting Disease tissue studied:  bone cancer, osteosarcoma Relevant cell lines - cell types - tissues:  293 (epithelial), MG63 (bone cell) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes WYE132 decrease temsirolimus decrease mTOR (human) increase Downstream Regulation Effect of modification (process):  transcription, induced

S68-p - MAF1 (human) ▲

Modsite: PPQtsGLsPsRLsks SwissProt Entrez-Gene Orthologous residues MAF1 (human): S68‑p, MAF1 (mouse): S68‑p, MAF1 (rat): S68‑p Characterization Methods used to characterize site in vivo:  electrophoretic mobility shift, mutation of modification site, western blotting Disease tissue studied:  bone cancer, osteosarcoma Relevant cell lines - cell types - tissues:  293 (epithelial), MG63 (bone cell) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes WYE132 decrease temsirolimus decrease mTOR (human) increase Downstream Regulation Effect of modification (process):  transcription, induced

S75-p - MAF1 (human) ▲

Modsite: sPsRLsksQGGEEEG SwissProt Entrez-Gene Orthologous residues MAF1 (human): S75‑p, MAF1 (mouse): S75‑p, MAF1 (rat): S75‑p Characterization Methods used to characterize site in vivo:  electrophoretic mobility shift, mass spectrometry, mutation of modification site, western blotting Disease tissue studied:  bone cancer, osteosarcoma Relevant cell lines - cell types - tissues:  293 (epithelial), MDA-MB-361 (breast cell), MG63 (bone cell) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes WYE132 decrease temsirolimus decrease mTOR (human) increase Downstream Regulation Effect of modification (process):  transcription, induced

T412-p - p70S6K (human) ▲

Modsite: NQVFLGFtyVAPsVL SwissProt Entrez-Gene Orthologous residues p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p Characterization Methods used to characterize site in vivo:  phospho-antibody, western blotting Disease tissue studied:  bone cancer, osteosarcoma Relevant cell lines - cell types - tissues:  MDA-MB-361 (breast cell), MG63 (bone cell) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes WYE132 decrease temsirolimus decrease temsirolimus decrease