Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.6.0.2
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 20169205
Gwinn DM, Asara JM, Shaw RJ (2010) Raptor is phosphorylated by cdc2 during mitosis. PLoS One 5, e9197 20169205
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

S696-p - Raptor (human)
Modsite: EKNyALPsPAttEGG SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S696‑p, Raptor (mouse): S696‑p, Raptor (rat): S696‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  lung cancer
Relevant cell lines - cell types - tissues:  A549 (pulmonary), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  in mitotic cells
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase
taxol increase

T706-p - Raptor (human)
Modsite: ttEGGsLtPVRDsPC SwissProt Entrez-Gene
Orthologous residues
Raptor (human): T706‑p, Raptor (mouse): T706‑p, Raptor (rat): T706‑p
Characterization
Methods used to characterize site in vivo electrophoretic mobility shift, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  lung cancer
Relevant cell lines - cell types - tissues:  A549 (pulmonary), HEK293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  in mitotic cells
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CDK1 (human) co-immunoprecipitation, pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nocodazole increase
seliciclib nocodazole inhibit treatment-induced increase

S711-p - Raptor (human)
Modsite: sLtPVRDsPCtPrLr SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S711‑p, Raptor (mouse): S711‑p, Raptor (rat): S711‑p

S719-p - Raptor (human)
Modsite: PCtPrLrsVssyGNI SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S719‑p, Raptor (mouse): S719‑p, Raptor (rat): S719‑p

S721-p - Raptor (human)
Modsite: tPrLrsVssyGNIRA SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S721‑p, Raptor (mouse): S721‑p, Raptor (rat): S721‑p

S722-p - Raptor (human)
Modsite: PrLrsVssyGNIRAV SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S722‑p, Raptor (mouse): S722‑p, Raptor (rat): S722‑p

Y723-p - Raptor (human)
Modsite: rLrsVssyGNIRAVA SwissProt Entrez-Gene
Orthologous residues
Raptor (human): Y723‑p, Raptor (mouse): Y723‑p, Raptor (rat): Y723‑p

S738-p - Raptor (human)
Modsite: TARSLNKsLQNLSLT SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S738‑p, Raptor (mouse): S738‑p, Raptor (rat): S738‑p

S771-p - Raptor (human)
Modsite: SASSTLGsPENEEHI SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S771‑p, Raptor (mouse): S771‑p, Raptor (rat): S771‑p

S792-p - Raptor (human)
Modsite: DKMRRAssYSsLNSL SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S792‑p, Raptor (mouse): S792‑p, Raptor (rat): S792‑p

S795-p - Raptor (human)
Modsite: RRAssYSsLNSLIGV SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S795‑p, Raptor (mouse): A795‑p, Raptor (rat): A795‑p

T853-p - Raptor (human)
Modsite: RPQRVLDtssLtQsA SwissProt Entrez-Gene
Orthologous residues
Raptor (human): T853‑p, Raptor (mouse): T853‑p, Raptor (rat): T853‑p

S855-p - Raptor (human)
Modsite: QRVLDtssLtQsAPA SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S855‑p, Raptor (mouse): S855‑p, Raptor (rat): S855‑p

T857-p - Raptor (human)
Modsite: VLDtssLtQsAPAsP SwissProt Entrez-Gene
Orthologous residues
Raptor (human): T857‑p, Raptor (mouse): T857‑p, Raptor (rat): T857‑p

S859-p - Raptor (human)
Modsite: DtssLtQsAPAsPtN SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S859‑p, Raptor (mouse): S859‑p, Raptor (rat): S859‑p

S863-p - Raptor (human)
Modsite: LtQsAPAsPtNkGVH SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S863‑p, Raptor (mouse): S863‑p, Raptor (rat): S863‑p

T865-p - Raptor (human)
Modsite: QsAPAsPtNkGVHIH SwissProt Entrez-Gene
Orthologous residues
Raptor (human): T865‑p, Raptor (mouse): T865‑p, Raptor (rat): T865‑p

S877-p - Raptor (human)
Modsite: HIHQAGGsPPAssts SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S877‑p, Raptor (mouse): S877‑p, Raptor (rat): S877‑p

S881-p - Raptor (human)
Modsite: AGGsPPAsstsSssL SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S881‑p, Raptor (mouse): S881‑p, Raptor (rat): S881‑p

T883-p - Raptor (human)
Modsite: GsPPAsstsSssLtN SwissProt Entrez-Gene
Orthologous residues
Raptor (human): T883‑p, Raptor (mouse): T883‑p, Raptor (rat): T883‑p

S886-p - Raptor (human)
Modsite: PAsstsSssLtNDVA SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S886‑p, Raptor (mouse): C886‑p, Raptor (rat): C886‑p

T889-p - Raptor (human)
Modsite: stsSssLtNDVAkQP SwissProt Entrez-Gene
Orthologous residues
Raptor (human): T889‑p, Raptor (mouse): T889‑p, Raptor (rat): T889‑p