Curated Information
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Home > Curated Information Page > PubMed Id: 20142099
Yamada E, et al. (2010) Fyn-dependent regulation of energy expenditure and body weight is mediated by tyrosine phosphorylation of LKB1. Cell Metab 11, 113-24 20142099
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S79-p - ACC1 (mouse)
Modsite: FHMRSsMsGLHLVKQ SwissProt Entrez-Gene
Orthologous residues
ACC1 (human): S80‑p, ACC1 iso2 (human): S22‑p, ACC1 iso4 (human): S117‑p, ACC1 (mouse): S79‑p, ACC1 iso2 (mouse): S117‑p, ACC1 (rat): S79‑p, ACC1 iso2 (rat): S79‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  muscle
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SU6656 increase

S212-p - ACC2 (mouse)
Modsite: PtMRPsMsGLHLVKR SwissProt Entrez-Gene
Orthologous residues
ACC2 (human): S222‑p, ACC2 (mouse): S212‑p, ACC2 (rat): S218‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  muscle
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SU6656 increase

T172-p - AMPKA2 (mouse)
Modsite: sDGEFLRtsCGsPNY SwissProt Entrez-Gene
Orthologous residues
AMPKA2 (human): T172‑p, AMPKA2 (mouse): T172‑p, AMPKA2 (rat): T172‑p, AMPKA2 (pig): T172‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  muscle
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SU6656 increase
LKB1 (mouse) increase LKB1 Y261/365F double mutant

Y60-p - LKB1 (mouse)
Modsite: DLLGEGSyGKVKEVL SwissProt Entrez-Gene
Orthologous residues
LKB1 (human): Y60‑p, LKB1 iso2 (human): Y60‑p, LKB1 (mouse): Y60‑p, LKB1 (rat): Y60‑p, LKB1 (pig):

Y156-p - LKB1 (mouse)
Modsite: PVCQAHGyFRQLIDG SwissProt Entrez-Gene
Orthologous residues
LKB1 (human): Y156‑p, LKB1 iso2 (human): Y156‑p, LKB1 (mouse): Y156‑p, LKB1 (rat): Y156‑p, LKB1 (pig): Y62‑p

Y166-p - LKB1 (mouse)
Modsite: QLIDGLEyLHSQGIV SwissProt Entrez-Gene
Orthologous residues
LKB1 (human): Y166‑p, LKB1 iso2 (human): Y166‑p, LKB1 (mouse): Y166‑p, LKB1 (rat): Y166‑p, LKB1 (pig): Y72‑p

Y261-p - LKB1 (mouse)
Modsite: PFEGDNIyKLFENIG SwissProt Entrez-Gene
Orthologous residues
LKB1 (human): Y261‑p, LKB1 iso2 (human): Y261‑p, LKB1 (mouse): Y261‑p, LKB1 (rat): Y261‑p, LKB1 (pig): Y167‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast), C2C12 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Fyn (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Fyn (human) transfection of inactive enzyme, transfection of constitutively active enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SU6656 decrease
Downstream Regulation
Effect of modification (function):  intracellular localization, phosphorylation
Comments:  mutation of this site increses AMPK phosphorylation

Y365-p - LKB1 (mouse)
Modsite: DIEDGIIytQDFTVP SwissProt Entrez-Gene
Orthologous residues
LKB1 (human): Y362‑p, LKB1 iso2 (human): Y362‑p, LKB1 (mouse): Y365‑p, LKB1 (rat): Y365‑p, LKB1 (pig): Y270‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast), C2C12 (myoblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Fyn (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Fyn (human) transfection of inactive enzyme, transfection of constitutively active enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SU6656 decrease
Downstream Regulation
Effect of modification (function):  intracellular localization, phosphorylation
Comments:  mutation of this site increses AMPK phosphorylation