Curated Information
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Home > Curated Information Page > PubMed Id: 20308788
Qian W, et al. (2010) PP2A regulates tau phosphorylation directly and also indirectly via activating GSK-3beta. J Alzheimers Dis 19, 1221-9 20308788
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S199-p - Tau iso8 (human)
Modsite: GDRsGyssPGsPGtP SwissProt Entrez-Gene
Orthologous residues
Tau (human): S516‑p, Tau iso2 (human): S141‑p, Tau iso3 (human): S105‑p, Tau iso4 (human): S170‑p, Tau iso5 (human): S199‑p, Tau iso6 (human): S141‑p, Tau iso7 (human): S170‑p, Tau iso8 (human): S199‑p, Tau (mouse): S491‑p, Tau iso3 (mouse): S188‑p, Tau iso7 (mouse): S148‑p, Tau (rat): S510‑p, Tau (cow): S206‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  Alzheimer's disease
Relevant cell lines - cell types - tissues:  CHO (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster
Enzymes shown to modify site in vitro
Type Enzyme
PHOSPHATASE PPP2CA (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) phospho-antibody, transfection of wild-type enzyme

S202-p - Tau iso8 (human)
Modsite: sGyssPGsPGtPGsR SwissProt Entrez-Gene
Orthologous residues
Tau (human): S519‑p, Tau iso2 (human): S144‑p, Tau iso3 (human): S108‑p, Tau iso4 (human): S173‑p, Tau iso5 (human): S202‑p, Tau iso6 (human): S144‑p, Tau iso7 (human): S173‑p, Tau iso8 (human): S202‑p, Tau (mouse): S494‑p, Tau iso3 (mouse): S191‑p, Tau iso7 (mouse): S151‑p, Tau (rat): S513‑p, Tau (cow): S209‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  Alzheimer's disease
Relevant cell lines - cell types - tissues:  CHO (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster
Enzymes shown to modify site in vitro
Type Enzyme
PHOSPHATASE PPP2CA (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) phospho-antibody, transfection of wild-type enzyme

T205-p - Tau iso8 (human)
Modsite: ssPGsPGtPGsRsRt SwissProt Entrez-Gene
Orthologous residues
Tau (human): T522‑p, Tau iso2 (human): T147‑p, Tau iso3 (human): T111‑p, Tau iso4 (human): T176‑p, Tau iso5 (human): T205‑p, Tau iso6 (human): T147‑p, Tau iso7 (human): T176‑p, Tau iso8 (human): T205‑p, Tau (mouse): T497‑p, Tau iso3 (mouse): T194‑p, Tau iso7 (mouse): T154‑p, Tau (rat): T516‑p, Tau (cow): T212‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  Alzheimer's disease
Relevant cell lines - cell types - tissues:  CHO (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster
Enzymes shown to modify site in vitro
Type Enzyme
PHOSPHATASE PPP2CA (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) phospho-antibody, transfection of wild-type enzyme

T212-p - Tau iso8 (human)
Modsite: tPGsRsRtPsLPtPP SwissProt Entrez-Gene
Orthologous residues
Tau (human): T529‑p, Tau iso2 (human): T154‑p, Tau iso3 (human): T118‑p, Tau iso4 (human): T183‑p, Tau iso5 (human): T212‑p, Tau iso6 (human): T154‑p, Tau iso7 (human): T183‑p, Tau iso8 (human): T212‑p, Tau (mouse): T504‑p, Tau iso3 (mouse): T201‑p, Tau iso7 (mouse): T161‑p, Tau (rat): T523‑p, Tau (cow): T219‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  Alzheimer's disease
Relevant cell lines - cell types - tissues:  CHO (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster
Enzymes shown to modify site in vitro
Type Enzyme
PHOSPHATASE PPP2CA (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) phospho-antibody, transfection of wild-type enzyme

S214-p - Tau iso8 (human)
Modsite: GsRsRtPsLPtPPtR SwissProt Entrez-Gene
Orthologous residues
Tau (human): S531‑p, Tau iso2 (human): S156‑p, Tau iso3 (human): S120‑p, Tau iso4 (human): S185‑p, Tau iso5 (human): S214‑p, Tau iso6 (human): S156‑p, Tau iso7 (human): S185‑p, Tau iso8 (human): S214‑p, Tau (mouse): S506‑p, Tau iso3 (mouse): S203‑p, Tau iso7 (mouse): S163‑p, Tau (rat): S525‑p, Tau (cow): S221‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  Alzheimer's disease
Relevant cell lines - cell types - tissues:  CHO (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster
Enzymes shown to modify site in vitro
Type Enzyme
PHOSPHATASE PPP2CA (human)

T217-p - Tau iso8 (human)
Modsite: sRtPsLPtPPtREPK SwissProt Entrez-Gene
Orthologous residues
Tau (human): T534‑p, Tau iso2 (human): T159‑p, Tau iso3 (human): T123‑p, Tau iso4 (human): T188‑p, Tau iso5 (human): T217‑p, Tau iso6 (human): T159‑p, Tau iso7 (human): T188‑p, Tau iso8 (human): T217‑p, Tau (mouse): T509‑p, Tau iso3 (mouse): T206‑p, Tau iso7 (mouse): T166‑p, Tau (rat): T528‑p, Tau (cow): T224‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  Alzheimer's disease
Relevant cell lines - cell types - tissues:  CHO (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) phospho-antibody, transfection of wild-type enzyme

S262-p - Tau iso8 (human)
Modsite: NVKsKIGstENLkHQ SwissProt Entrez-Gene
Orthologous residues
Tau (human): S579‑p, Tau iso2 (human): S204‑p, Tau iso3 (human): S168‑p, Tau iso4 (human): S233‑p, Tau iso5 (human): S262‑p, Tau iso6 (human): S204‑p, Tau iso7 (human): S233‑p, Tau iso8 (human): S262‑p, Tau (mouse): S554‑p, Tau iso3 (mouse): S251‑p, Tau iso7 (mouse): S211‑p, Tau (rat): S573‑p, Tau (cow): S269‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  Alzheimer's disease
Relevant cell lines - cell types - tissues:  CHO (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster
Enzymes shown to modify site in vitro
Type Enzyme
PHOSPHATASE PPP2CA (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) phospho-antibody, transfection of wild-type enzyme

S396-p - Tau iso8 (human)
Modsite: GAEIVyKsPVVsGDt SwissProt Entrez-Gene
Orthologous residues
Tau (human): S713‑p, Tau iso2 (human): S307‑p, Tau iso3 (human): S271‑p, Tau iso4 (human): S336‑p, Tau iso5 (human): S365‑p, Tau iso6 (human): S338‑p, Tau iso7 (human): S367‑p, Tau iso8 (human): S396‑p, Tau (mouse): S688‑p, Tau iso3 (mouse): S385‑p, Tau iso7 (mouse): S345‑p, Tau (rat): S707‑p, Tau (cow): S403‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  Alzheimer's disease
Relevant cell lines - cell types - tissues:  CHO (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster
Enzymes shown to modify site in vitro
Type Enzyme
PHOSPHATASE PPP2CA (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) phospho-antibody, transfection of wild-type enzyme

S404-p - Tau iso8 (human)
Modsite: PVVsGDtsPRHLsNV SwissProt Entrez-Gene
Orthologous residues
Tau (human): S721‑p, Tau iso2 (human): S315‑p, Tau iso3 (human): S279‑p, Tau iso4 (human): S344‑p, Tau iso5 (human): S373‑p, Tau iso6 (human): S346‑p, Tau iso7 (human): S375‑p, Tau iso8 (human): S404‑p, Tau (mouse): S696‑p, Tau iso3 (mouse): S393‑p, Tau iso7 (mouse): S353‑p, Tau (rat): S715‑p, Tau (cow): S411‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  Alzheimer's disease
Relevant cell lines - cell types - tissues:  CHO (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster
Enzymes shown to modify site in vitro
Type Enzyme
PHOSPHATASE PPP2CA (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE GSK3B (human) phospho-antibody, transfection of wild-type enzyme

S409-p - Tau iso8 (human)
Modsite: DtsPRHLsNVsstGs SwissProt Entrez-Gene
Orthologous residues
Tau (human): S726‑p, Tau iso2 (human): S320‑p, Tau iso3 (human): S284‑p, Tau iso4 (human): S349‑p, Tau iso5 (human): S378‑p, Tau iso6 (human): S351‑p, Tau iso7 (human): S380‑p, Tau iso8 (human): S409‑p, Tau (mouse): S701‑p, Tau iso3 (mouse): S398‑p, Tau iso7 (mouse): S358‑p, Tau (rat): S720‑p, Tau (cow): S416‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  Alzheimer's disease
Relevant cell lines - cell types - tissues:  CHO (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster
Enzymes shown to modify site in vitro
Type Enzyme
PHOSPHATASE PPP2CA (human)

T308-p - Akt1 (rat)
Modsite: KDGATMKtFCGTPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  Alzheimer's disease
Relevant cell lines - cell types - tissues:  brain
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
okadaic_acid increase

S473-p - Akt1 (rat)
Modsite: RPHFPQFsYSASGTA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  Alzheimer's disease
Relevant cell lines - cell types - tissues:  brain
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
okadaic_acid increase

S9-p - GSK3B (rat)
Modsite: SGRPRTTsFAESCKP SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S9‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  Alzheimer's disease
Relevant cell lines - cell types - tissues:  brain
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
okadaic_acid increase

S510-p - Tau (rat)
Modsite: GERSGyssPGsPGtP SwissProt Entrez-Gene
Orthologous residues
Tau (human): S516‑p, Tau iso2 (human): S141‑p, Tau iso3 (human): S105‑p, Tau iso4 (human): S170‑p, Tau iso5 (human): S199‑p, Tau iso6 (human): S141‑p, Tau iso7 (human): S170‑p, Tau iso8 (human): S199‑p, Tau (mouse): S491‑p, Tau iso3 (mouse): S188‑p, Tau iso7 (mouse): S148‑p, Tau (rat): S510‑p, Tau (cow): S206‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  Alzheimer's disease
Relevant cell lines - cell types - tissues:  brain
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
okadaic_acid decrease

S513-p - Tau (rat)
Modsite: SGyssPGsPGtPGsR SwissProt Entrez-Gene
Orthologous residues
Tau (human): S519‑p, Tau iso2 (human): S144‑p, Tau iso3 (human): S108‑p, Tau iso4 (human): S173‑p, Tau iso5 (human): S202‑p, Tau iso6 (human): S144‑p, Tau iso7 (human): S173‑p, Tau iso8 (human): S202‑p, Tau (mouse): S494‑p, Tau iso3 (mouse): S191‑p, Tau iso7 (mouse): S151‑p, Tau (rat): S513‑p, Tau (cow): S209‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  Alzheimer's disease
Relevant cell lines - cell types - tissues:  brain
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
okadaic_acid increase

T516-p - Tau (rat)
Modsite: ssPGsPGtPGsRSRt SwissProt Entrez-Gene
Orthologous residues
Tau (human): T522‑p, Tau iso2 (human): T147‑p, Tau iso3 (human): T111‑p, Tau iso4 (human): T176‑p, Tau iso5 (human): T205‑p, Tau iso6 (human): T147‑p, Tau iso7 (human): T176‑p, Tau iso8 (human): T205‑p, Tau (mouse): T497‑p, Tau iso3 (mouse): T194‑p, Tau iso7 (mouse): T154‑p, Tau (rat): T516‑p, Tau (cow): T212‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  Alzheimer's disease
Relevant cell lines - cell types - tissues:  brain
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
okadaic_acid increase

T523-p - Tau (rat)
Modsite: tPGsRSRtPsLPtPP SwissProt Entrez-Gene
Orthologous residues
Tau (human): T529‑p, Tau iso2 (human): T154‑p, Tau iso3 (human): T118‑p, Tau iso4 (human): T183‑p, Tau iso5 (human): T212‑p, Tau iso6 (human): T154‑p, Tau iso7 (human): T183‑p, Tau iso8 (human): T212‑p, Tau (mouse): T504‑p, Tau iso3 (mouse): T201‑p, Tau iso7 (mouse): T161‑p, Tau (rat): T523‑p, Tau (cow): T219‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  Alzheimer's disease
Relevant cell lines - cell types - tissues:  brain
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
okadaic_acid increase

S525-p - Tau (rat)
Modsite: GsRSRtPsLPtPPTR SwissProt Entrez-Gene
Orthologous residues
Tau (human): S531‑p, Tau iso2 (human): S156‑p, Tau iso3 (human): S120‑p, Tau iso4 (human): S185‑p, Tau iso5 (human): S214‑p, Tau iso6 (human): S156‑p, Tau iso7 (human): S185‑p, Tau iso8 (human): S214‑p, Tau (mouse): S506‑p, Tau iso3 (mouse): S203‑p, Tau iso7 (mouse): S163‑p, Tau (rat): S525‑p, Tau (cow): S221‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  Alzheimer's disease
Relevant cell lines - cell types - tissues:  brain
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
okadaic_acid increase

T528-p - Tau (rat)
Modsite: SRtPsLPtPPTREPK SwissProt Entrez-Gene
Orthologous residues
Tau (human): T534‑p, Tau iso2 (human): T159‑p, Tau iso3 (human): T123‑p, Tau iso4 (human): T188‑p, Tau iso5 (human): T217‑p, Tau iso6 (human): T159‑p, Tau iso7 (human): T188‑p, Tau iso8 (human): T217‑p, Tau (mouse): T509‑p, Tau iso3 (mouse): T206‑p, Tau iso7 (mouse): T166‑p, Tau (rat): T528‑p, Tau (cow): T224‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  Alzheimer's disease
Relevant cell lines - cell types - tissues:  brain
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
okadaic_acid increase

S573-p - Tau (rat)
Modsite: NVRSKIGsTENLKHQ SwissProt Entrez-Gene
Orthologous residues
Tau (human): S579‑p, Tau iso2 (human): S204‑p, Tau iso3 (human): S168‑p, Tau iso4 (human): S233‑p, Tau iso5 (human): S262‑p, Tau iso6 (human): S204‑p, Tau iso7 (human): S233‑p, Tau iso8 (human): S262‑p, Tau (mouse): S554‑p, Tau iso3 (mouse): S251‑p, Tau iso7 (mouse): S211‑p, Tau (rat): S573‑p, Tau (cow): S269‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  Alzheimer's disease
Relevant cell lines - cell types - tissues:  brain
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
okadaic_acid increase

S707-p - Tau (rat)
Modsite: GAEIVyKsPVVsGDt SwissProt Entrez-Gene
Orthologous residues
Tau (human): S713‑p, Tau iso2 (human): S307‑p, Tau iso3 (human): S271‑p, Tau iso4 (human): S336‑p, Tau iso5 (human): S365‑p, Tau iso6 (human): S338‑p, Tau iso7 (human): S367‑p, Tau iso8 (human): S396‑p, Tau (mouse): S688‑p, Tau iso3 (mouse): S385‑p, Tau iso7 (mouse): S345‑p, Tau (rat): S707‑p, Tau (cow): S403‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  Alzheimer's disease
Relevant cell lines - cell types - tissues:  brain
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
okadaic_acid no change compared to control

S715-p - Tau (rat)
Modsite: PVVsGDtsPRHLSNV SwissProt Entrez-Gene
Orthologous residues
Tau (human): S721‑p, Tau iso2 (human): S315‑p, Tau iso3 (human): S279‑p, Tau iso4 (human): S344‑p, Tau iso5 (human): S373‑p, Tau iso6 (human): S346‑p, Tau iso7 (human): S375‑p, Tau iso8 (human): S404‑p, Tau (mouse): S696‑p, Tau iso3 (mouse): S393‑p, Tau iso7 (mouse): S353‑p, Tau (rat): S715‑p, Tau (cow): S411‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  Alzheimer's disease
Relevant cell lines - cell types - tissues:  brain
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
okadaic_acid increase

S733-p - Tau (rat)
Modsite: GsIDMVDsPQLATLA SwissProt Entrez-Gene
Orthologous residues
Tau (human): S739‑p, Tau iso2 (human): S333‑p, Tau iso3 (human): S297‑p, Tau iso4 (human): S362‑p, Tau iso5 (human): S391‑p, Tau iso6 (human): S364‑p, Tau iso7 (human): S393‑p, Tau iso8 (human): S422‑p, Tau (mouse): S714‑p, Tau iso3 (mouse): S411‑p, Tau iso7 (mouse): S371‑p, Tau (rat): S733‑p, Tau (cow): S429‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  Alzheimer's disease
Relevant cell lines - cell types - tissues:  brain
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
okadaic_acid increase