Curated Information
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Home > Curated Information Page > PubMed Id: 20227368
Yan L, et al. (2010) PP2A(T61epsilon) Is an Inhibitor of MAP4K3 in Nutrient Signaling to mTOR. Mol Cell 37, 633-642 20227368
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S170-p - KHS2 (human)
Modsite: AtIAkRKsFIGTPYW SwissProt Entrez-Gene
Orthologous residues
KHS2 (human): S170‑p, KHS2 iso2 (human): S160‑p, KHS2 iso3 (human): S170‑p, KHS2 (mouse): S170‑p, KHS2 iso100 (mouse): S170‑p, KHS2 (rat): S170‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody
Relevant cell lines - cell types - tissues:  293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE KHS2 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PPP2CA (human)
KINASE KHS2 (human) transfection of wild-type enzyme, transfection of inactive enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
amino_acids increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S398-p - KHS2 (human)
Modsite: ANKSLLksVEEELHQ SwissProt Entrez-Gene
Orthologous residues
KHS2 (human): S398‑p, KHS2 iso2 (human): S388‑p, KHS2 iso3 (human): S377‑p, KHS2 (mouse): S398‑p, KHS2 iso100 (mouse): S398‑p, KHS2 (rat): S377‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

T412-p - p70S6K (human)
Modsite: NQVFLGFtyVAPsVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
KHS2 (human) increase

S240-p - S6 (human)
Modsite: RLssLRAstsKsEss SwissProt Entrez-Gene
Orthologous residues
S6 (human): S240‑p, S6 (mouse): S240‑p, S6 (rat): S240‑p, S6 (fruit fly): S239‑p, S6 (cow): S240‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
KHS2 (human) increase

S244-p - S6 (human)
Modsite: LRAstsKsEssQK__ SwissProt Entrez-Gene
Orthologous residues
S6 (human): S244‑p, S6 (mouse): S244‑p, S6 (rat): S244‑p, S6 (fruit fly): V243‑p, S6 (cow): S244‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  293T (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
KHS2 (human) increase