Curated Information
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Home > Curated Information Page > PubMed Id: 10480933
Jensen CJ, et al. (1999) 90-kDa ribosomal S6 kinase is phosphorylated and activated by 3-phosphoinositide-dependent protein kinase-1. J Biol Chem 274, 27168-76 10480933
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S227-p - RSK2 (mouse)
Modsite: DHEKKAysFCGtVEy SwissProt Entrez-Gene
Orthologous residues
RSK2 (human): S227‑p, RSK2 (mouse): S227‑p, RSK2 (rat): S227‑p, RSK2 (cow): S227‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PDK1 (mouse)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S369-p - RSK2 (mouse)
Modsite: TAKtPKDsPGIPPsA SwissProt Entrez-Gene
Orthologous residues
RSK2 (human): S369‑p, RSK2 (mouse): S369‑p, RSK2 (rat): S369‑p, RSK2 (cow): S369‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PDK1 (mouse)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S386-p - RSK2 (mouse)
Modsite: HQLFRGFsFVAITSD SwissProt Entrez-Gene
Orthologous residues
RSK2 (human): S386‑p, RSK2 (mouse): S386‑p, RSK2 (rat): S386‑p, RSK2 (cow): S386‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK1 (mouse)
KINASE PDK1 (mouse)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

T577-p - RSK2 (mouse)
Modsite: AENGLLMtPCYtANF SwissProt Entrez-Gene
Orthologous residues
RSK2 (human): T577‑p, RSK2 (mouse): T577‑p, RSK2 (rat): T577‑p, RSK2 (cow): T577‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  COS (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PDK1 (mouse)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
EGF increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced