Curated Information
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Home > Curated Information Page > PubMed Id: 30872361
Freitas-Andrade M, et al. (2019) Targeting MAPK phosphorylation of Connexin43 provides neuroprotection in stroke. J Exp Med 216, 916-935 30872361
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S255-p - GJA1 (mouse)
Modsite: HAtTGPLsPskDCGs SwissProt Entrez-Gene
Orthologous residues
GJA1 (human): S255‑p, GJA1 (mouse): S255‑p, GJA1 (rat): S255‑p, GJA1 (rabbit): S255‑p, GJA1 (pig): S255‑p, GJA1 (hamster): N255‑p, GJA1 (cow): S256‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  brain
Cellular systems studied:  tissue
Species studied:  mouse

S262-p - GJA1 (mouse)
Modsite: sPskDCGsPkyAyFN SwissProt Entrez-Gene
Orthologous residues
GJA1 (human): S262‑p, GJA1 (mouse): S262‑p, GJA1 (rat): S262‑p, GJA1 (rabbit): S262‑p, GJA1 (pig): S262‑p, GJA1 (hamster): S262‑p, GJA1 (cow): S263‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  brain
Cellular systems studied:  tissue
Species studied:  mouse

S279-p - GJA1 (mouse)
Modsite: SsPtAPLsPMsPPGy SwissProt Entrez-Gene
Orthologous residues
GJA1 (human): S279‑p, GJA1 (mouse): S279‑p, GJA1 (rat): S279‑p, GJA1 (rabbit): S279‑p, GJA1 (pig): S279‑p, GJA1 (hamster): S279‑p, GJA1 (cow): S280‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  brain
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
normoxia increase
hypoxia increase
Downstream Regulation
Effect of modification (function):  activity, induced
Comments:  induce loop tail interaction which enhances/regulates Cx43 hemichannel function (hemichannel opening)

S282-p - GJA1 (mouse)
Modsite: tAPLsPMsPPGykLV SwissProt Entrez-Gene
Orthologous residues
GJA1 (human): S282‑p, GJA1 (mouse): S282‑p, GJA1 (rat): S282‑p, GJA1 (rabbit): S282‑p, GJA1 (pig): S282‑p, GJA1 (hamster): S282‑p, GJA1 (cow): S283‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  brain
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
normoxia increase
hypoxia increase
Downstream Regulation
Effect of modification (function):  activity, induced
Comments:  induce loop tail interaction which enhances/regulates Cx43 hemichannel function (hemichannel opening)

S325-p - GJA1 (mouse)
Modsite: NRMGQAGstIsNsHA SwissProt Entrez-Gene
Orthologous residues
GJA1 (human): S325‑p, GJA1 (mouse): S325‑p, GJA1 (rat): S325‑p, GJA1 (rabbit): S325‑p, GJA1 (pig): S325‑p, GJA1 (hamster): S325‑p, GJA1 (cow): S326‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  brain
Cellular systems studied:  tissue
Species studied:  mouse

S328-p - GJA1 (mouse)
Modsite: GQAGstIsNsHAQPF SwissProt Entrez-Gene
Orthologous residues
GJA1 (human): S328‑p, GJA1 (mouse): S328‑p, GJA1 (rat): S328‑p, GJA1 (rabbit): S328‑p, GJA1 (pig): S328‑p, GJA1 (hamster): S328‑p, GJA1 (cow): S329‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  brain
Cellular systems studied:  tissue
Species studied:  mouse

S330-p - GJA1 (mouse)
Modsite: AGstIsNsHAQPFDF SwissProt Entrez-Gene
Orthologous residues
GJA1 (human): S330‑p, GJA1 (mouse): S330‑p, GJA1 (rat): S330‑p, GJA1 (rabbit): S330‑p, GJA1 (pig): S330‑p, GJA1 (hamster): S330‑p, GJA1 (cow): S331‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  brain
Cellular systems studied:  tissue
Species studied:  mouse

S368-p - GJA1 (mouse)
Modsite: QRPssRAssRAssRP SwissProt Entrez-Gene
Orthologous residues
GJA1 (human): S368‑p, GJA1 (mouse): S368‑p, GJA1 (rat): S368‑p, GJA1 (rabbit): S368‑p, GJA1 (pig): S368‑p, GJA1 (hamster): S368‑p, GJA1 (cow): S369‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  brain
Cellular systems studied:  tissue
Species studied:  mouse