Curated Information
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Home > Curated Information Page > PubMed Id: 19713527
Yang WL, et al. (2009) The E3 ligase TRAF6 regulates Akt ubiquitination and activation. Science 325, 1134-8 19713527
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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K8-ub - Akt1 (human)
Modsite: MsDVAIVkEGWLHkR SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): K8‑ub, Akt1 iso2 (human): , Akt1 (mouse): K8‑ub, Akt1 (rat): K8‑ub, Akt1 (fruit fly): K109‑ub, Akt1 (cow): K8‑ub
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
UBIQUITIN LIGASE TRAF6 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE TRAF6 (human) transfection of inactive enzyme, transfection of wild-type enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IGF-1 increase
serum increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, intracellular localization, phosphorylation
Comments:  promotes Akt membrane recruitment and phosphorylation, resulting in activation

K14-ub - Akt1 (human)
Modsite: VkEGWLHkRGEYIkT SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): K14‑ub, Akt1 iso2 (human): , Akt1 (mouse): K14‑ub, Akt1 (rat): K14‑ub, Akt1 (fruit fly): K115‑ub, Akt1 (cow): K14‑ub
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
UBIQUITIN LIGASE TRAF6 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE TRAF6 (human) transfection of inactive enzyme, transfection of wild-type enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IGF-1 increase
serum increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, intracellular localization, phosphorylation
Comments:  promotes Akt membrane recruitment and phosphorylation, resulting in activation

T308-p - Akt1 (human)
Modsite: kDGAtMKtFCGtPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TRAF6 (human) increase
LPS, serum TRAF6 (human) increase
IL-1b, serum TRAF6 (human) increase
adriamycin TRAF6 (human) increase
cisplatin TRAF6 (human) increase
IGF-1 TRAF6 (human) increase
serum TRAF6 (human) increase

S473-p - Akt1 (human)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
IGF-1 TRAF6 (human) increase
serum TRAF6 (human) increase