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Home > Curated Information Page > PubMed Id: 17210579
Takeda K, et al. (2007) Apoptosis signal-regulating kinase (ASK) 2 functions as a mitogen-activated protein kinase kinase kinase in a heteromeric complex with ASK1. J Biol Chem 282, 7522-31 17210579
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T183-p - JNK1 (human)
Modsite: AGtsFMMtPyVVtRY SwissProt Entrez-Gene
Orthologous residues
JNK1 (human): T183‑p, JNK1 iso2 (human): T183‑p, JNK1 iso3 (human): T183‑p, JNK1 (mouse): T183‑p, JNK1 (rat): T183‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  melanoma skin cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), B16 (melanocyte)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MEKK6 (human) transfection of wild-type enzyme, siRNA inhibition of enzyme, transfection of inactive enzyme, phospho-antibody phosphorylates in heteromeric complex with ASK1
KINASE MEKK6 (human) transfection of wild-type enzyme, transfection of inactive enzyme
Comments:  MEKK6 autophosphorylates in the presence of ASK1.
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H2O2 increase
ASK1 (human) increase ASK1 siRNA decreases
MEKK6 (human) increase MEKK6 siRNA decreases

Y185-p - JNK1 (human)
Modsite: tsFMMtPyVVtRYYR SwissProt Entrez-Gene
Orthologous residues
JNK1 (human): Y185‑p, JNK1 iso2 (human): Y185‑p, JNK1 iso3 (human): Y185‑p, JNK1 (mouse): Y185‑p, JNK1 (rat): Y185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  melanoma skin cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), B16 (melanocyte)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MEKK6 (human) transfection of wild-type enzyme, siRNA inhibition of enzyme, transfection of inactive enzyme, phospho-antibody phosphorylates in heteromeric complex with ASK1
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H2O2 increase
ASK1 (human) increase ASK1 siRNA decreases
MEKK6 (human) increase MEKK6 siRNA decreases

T806-p - MEKK6 (human)
Modsite: GItPCTEtFTGTLQY SwissProt Entrez-Gene
Orthologous residues
MEKK6 (human): T806‑p, MEKK6 iso2 (human): T529‑p, MEKK6 iso3 (human): T798‑p, MEKK6 (mouse): T807‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), E.coli (bacterial)
Cellular systems studied:  cell lines
Species studied:  bacteria, human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE MEKK6 (human)
Comments:  MEKK6 is autophosphorylated in the presence of ASK1.
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MEKK6 (human) transfection of wild-type enzyme, transfection of inactive enzyme
Comments:  MEKK6 autophosphorylates in the presence of ASK1.
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
H2O2 ASK1 (human) increase

T180-p - P38A (human)
Modsite: RHtDDEMtGyVAtRW SwissProt Entrez-Gene
Orthologous residues
P38A (human): T180‑p, P38A iso2 (human): T180‑p, P38A (mouse): T180‑p, P38A iso3 (mouse): T180‑p, P38A (rat): T180‑p, P38A (salmonid): T181‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  melanoma skin cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), B16 (melanocyte)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MEKK6 (human) transfection of wild-type enzyme, siRNA inhibition of enzyme, transfection of inactive enzyme, phospho-antibody phosphorylates in heteromeric complex with ASK1

Y182-p - P38A (human)
Modsite: tDDEMtGyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
P38A (human): Y182‑p, P38A iso2 (human): Y182‑p, P38A (mouse): Y182‑p, P38A iso3 (mouse): Y182‑p, P38A (rat): Y182‑p, P38A (salmonid): Y183‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  melanoma skin cancer
Relevant cell lines - cell types - tissues:  293 (epithelial), B16 (melanocyte)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MEKK6 (human) transfection of wild-type enzyme, siRNA inhibition of enzyme, transfection of inactive enzyme, phospho-antibody phosphorylates in heteromeric complex with ASK1

T845-p - ASK1 (mouse)
Modsite: GINPCtEtFTGtLQY SwissProt Entrez-Gene
Orthologous residues
ASK1 (human): T838‑p, ASK1 iso5 (human): T918‑p, ASK1 (mouse): T845‑p, ASK1 (rat): T844‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), E.coli (bacterial)
Cellular systems studied:  cell lines
Species studied:  bacteria, human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MEKK6 (human) transfection of wild-type enzyme, transfection of inactive enzyme, phospho-antibody
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

T807-p - MEKK6 (mouse)
Modsite: GITPCTEtFTGTLQY SwissProt Entrez-Gene
Orthologous residues
MEKK6 (human): T806‑p, MEKK6 iso2 (human): T529‑p, MEKK6 iso3 (human): T798‑p, MEKK6 (mouse): T807‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), E.coli (bacterial)
Cellular systems studied:  cell lines
Species studied:  bacteria, human