Curated Information
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Home > Curated Information Page > PubMed Id: 18449195
Richard DJ, et al. (2008) Single-stranded DNA-binding protein hSSB1 is critical for genomic stability. Nature 453, 677-81 18449195
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S1981-p - ATM (human)
Modsite: sLAFEEGsQSTtIss SwissProt Entrez-Gene
Orthologous residues
ATM (human): S1981‑p, ATM (mouse): S1987‑p, ATM (rat): S1988‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  fibroblast-foreskin
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation OBFC2B (human) increase OBFC2B/SSB1 siRNA reduces induction
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S317-p - Chk1 (human)
Modsite: ENVkYsssQPEPRTG SwissProt Entrez-Gene
Orthologous residues
Chk1 (human): S317‑p, Chk1 (mouse): S317‑p, Chk1 (rat): S317‑p, Chk1 (chicken): S317‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  fibroblast-foreskin
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation OBFC2B (human) increase OBFC2B/SSB1 siRNA reduces induction

T68-p - Chk2 (human)
Modsite: SsLEtVstQELYsIP SwissProt Entrez-Gene
Orthologous residues
Chk2 (human): T68‑p, Chk2 iso9 (human): T68‑p, Chk2 iso11 (human): T68‑p, Chk2 iso12 (human): T68‑p, Chk2 (mouse): T77‑p, Chk2 (rat): T76‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  fibroblast-foreskin
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation OBFC2B (human) increase OBFC2B/SSB1 siRNA reduces induction

S343-p - NBS1 (human)
Modsite: TtPGPsLsQGVsVDE SwissProt Entrez-Gene
Orthologous residues
NBS1 (human): S343‑p, NBS1 (mouse): S343‑p, NBS1 (rat): S343‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  fibroblast-foreskin
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation OBFC2B (human) increase OBFC2B/SSB1 siRNA reduces induction

T117-p - OBFC2B (human)
Modsite: EPNPEYstQQAPNKA SwissProt Entrez-Gene
Orthologous residues
OBFC2B (human): T117‑p, OBFC2B (mouse): T117‑p, OBFC2B (rat): T117‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  fibroblast-foreskin
Cellular systems studied:  cell lines, primary cells
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ATM (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation OBFC2B (human) increase OBFC2B/SSB1 siRNA reduces induction

S15-p - p53 (human)
Modsite: PsVEPPLsQEtFsDL SwissProt Entrez-Gene
Orthologous residues
p53 (human): S15‑p, p53 iso2 (human): S15‑p, p53 iso4 (human): , p53 (mouse): S18‑p, p53 iso2 (mouse): S18‑p, p53 (rat): S15‑p, p53 (rabbit): S15‑p, p53 (green monkey): S15‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  fibroblast-foreskin
Cellular systems studied:  cell lines
Species studied:  human