Curated Information
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Home > Curated Information Page > PubMed Id: 17303383
Schroder WA, et al. (2007) Human Sin1 contains Ras-binding and pleckstrin homology domains and suppresses Ras signalling. Cell Signal 19, 1279-89 17303383
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S473-p - Akt1 (human)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  BHK (fibroblast) [HRas (human)], BHK (fibroblast) [KRas (human)], BHK (fibroblast) [Sin1 (human)]
Cellular systems studied:  cell lines
Species studied:  hamster
Comments:  Sin1 PH and RBD domains are divergent from prototypical domains and are not automatically predicted. Overexpression of Sin1 inhibited phosphorylation of this site by activated Ras. This observation was made in hamster cells with transfected human Sin1.

T202-p - ERK1 (human)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  BHK (fibroblast) [HRas (human)], BHK (fibroblast) [KRas (human)], BHK (fibroblast) [Sin1 (human)]
Cellular systems studied:  cell lines
Species studied:  hamster
Comments:  Sin1 PH and RBD domains are divergent from prototypical domains and are not automatically predicted. Overexpression of Sin1 inhibited phosphorylation of this site by activated Ras. This observation was made in hamster cells with transfected human Sin1. siRNA knockdown of endogenous Sin1 protein expression in HEK293 cells enhanced the phosphorylation of this site by activated Ras.

Y204-p - ERK1 (human)
Modsite: HtGFLtEyVAtRWyr SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  BHK (fibroblast) [HRas (human)], BHK (fibroblast) [KRas (human)], BHK (fibroblast) [Sin1 (human)]
Cellular systems studied:  cell lines
Species studied:  hamster
Comments:  Sin1 PH and RBD domains are divergent from prototypical domains and are not automatically predicted. Overexpression of Sin1 inhibited phosphorylation of this site by activated Ras. This observation was made in hamster cells with transfected human Sin1. siRNA knockdown of endogenous Sin1 protein expression in HEK293 cells enhanced the phosphorylation of this site by activated Ras.

T185-p - ERK2 (human)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  BHK (fibroblast) [HRas (human)], BHK (fibroblast) [KRas (human)], BHK (fibroblast) [Sin1 (human)]
Cellular systems studied:  cell lines
Species studied:  hamster
Comments:  Sin1 PH and RBD domains are divergent from prototypical domains and are not automatically predicted. Overexpression of Sin1 inhibited phosphorylation of this site by activated Ras. This observation was made in hamster cells with transfected human Sin1. siRNA knockdown of endogenous Sin1 protein expression in HEK293 cells enhanced the phosphorylation of this site by activated Ras.

Y187-p - ERK2 (human)
Modsite: HtGFLtEyVAtRWyr SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  BHK (fibroblast) [HRas (human)], BHK (fibroblast) [KRas (human)], BHK (fibroblast) [Sin1 (human)]
Cellular systems studied:  cell lines
Species studied:  hamster
Comments:  Sin1 PH and RBD domains are divergent from prototypical domains and are not automatically predicted. Overexpression of Sin1 inhibited phosphorylation of this site by activated Ras. This observation was made in hamster cells with transfected human Sin1. siRNA knockdown of endogenous Sin1 protein expression in HEK293 cells enhanced the phosphorylation of this site by activated Ras.

T183-p - JNK1 (human)
Modsite: AGtsFMMtPyVVtRY SwissProt Entrez-Gene
Orthologous residues
JNK1 (human): T183‑p, JNK1 iso2 (human): T183‑p, JNK1 iso3 (human): T183‑p, JNK1 (mouse): T183‑p, JNK1 (rat): T183‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  BHK (fibroblast) [HRas (human)], BHK (fibroblast) [KRas (human)], BHK (fibroblast) [Sin1 (human)]
Cellular systems studied:  cell lines
Species studied:  hamster
Comments:  Sin1 PH and RBD domains are divergent from prototypical domains and are not automatically predicted. Overexpression of Sin1 inhibited phosphorylation of this site by activated Ras. This observation was made in hamster cells with transfected human Sin1.

Y185-p - JNK1 (human)
Modsite: tsFMMtPyVVtRYYR SwissProt Entrez-Gene
Orthologous residues
JNK1 (human): Y185‑p, JNK1 iso2 (human): Y185‑p, JNK1 iso3 (human): Y185‑p, JNK1 (mouse): Y185‑p, JNK1 (rat): Y185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  BHK (fibroblast) [HRas (human)], BHK (fibroblast) [KRas (human)], BHK (fibroblast) [Sin1 (human)]
Cellular systems studied:  cell lines
Species studied:  hamster
Comments:  Sin1 PH and RBD domains are divergent from prototypical domains and are not automatically predicted. Overexpression of Sin1 inhibited phosphorylation of this site by activated Ras. This observation was made in hamster cells with transfected human Sin1.