Curated Information
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Home > Curated Information Page > PubMed Id: 17396146
Raman M, et al. (2007) TAO kinases mediate activation of p38 in response to DNA damage. EMBO J 26, 2005-14 17396146
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y15-p - CDK1 (human)
Modsite: EkIGEGtyGVVykGR SwissProt Entrez-Gene
Orthologous residues
CDK1 (human): Y15‑p, CDK1 iso2 (human): Y15‑p, CDK1 (mouse): Y15‑p, CDK1 (rat): Y15‑p, CDK1 (chicken): Y15‑p, CDK1 (fruit fly): Y15‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hydroxyurea inhibit treatment-induced increase
siRNA hydroxyurea TAO1 (human) inhibit treatment-induced increase
siRNA hydroxyurea TAO3 (human) inhibit treatment-induced increase
UV increase
siRNA UV TAO1 (human) inhibit treatment-induced increase
siRNA UV TAO3 (human) inhibit treatment-induced increase

S10-p - H3 (human)
Modsite: tkQtArkstGGkAPr SwissProt Entrez-Gene
Orthologous residues
H3 (human): S10‑p, H3 (mouse): S10‑p, H3 iso2 (mouse): S10‑p, H3 iso3 (mouse): S10‑p, H3 (rat): S10‑p, H3 iso3 (rat): S10‑p, H3 (pig): S10‑p, H3 (chicken): S10‑p, H3 (cow): S10‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
UV decrease
siRNA UV TAO3 (human) inhibit treatment-induced decrease
siRNA UV TAO1 (human) inhibit treatment-induced decrease
siRNA siRNA TAO3 (human) TAO1 (human) augment treatment-induced increase

T502-p - TAO1 (human)
Modsite: RLDKDLEtQRNNFAA SwissProt Entrez-Gene
Orthologous residues
TAO1 (human): T502‑p, TAO1 iso3 (human): T502‑p, TAO1 (mouse): T502‑p, TAO1 (rat): T502‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  activity, induced
Effect of modification (process):  cell cycle regulation
Comments:  activation of p38

S554-p - TAO1 (human)
Modsite: ELNSFLEsQKREYKL SwissProt Entrez-Gene
Orthologous residues
TAO1 (human): S554‑p, TAO1 iso3 (human): S554‑p, TAO1 (mouse): S554‑p, TAO1 (rat): S554‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human

T643-p - TAO1 (human)
Modsite: EELNKRQtQKDLEHA SwissProt Entrez-Gene
Orthologous residues
TAO1 (human): T643‑p, TAO1 iso3 (human): , TAO1 (mouse): T643‑p, TAO1 (rat): T643‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ATM (human)
Downstream Regulation
Effect of modification (function):  activity, induced
Effect of modification (process):  cell cycle regulation
Comments:  activation of p38

T785-p - TAO1 (human)
Modsite: SINEMLStQALRLDE SwissProt Entrez-Gene
Orthologous residues
TAO1 (human): T785‑p, TAO1 iso3 (human): T637‑p, TAO1 (mouse): T785‑p, TAO1 (rat): T785‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ATM (human)
Downstream Regulation
Effect of modification (function):  activity, induced
Effect of modification (process):  cell cycle regulation
Comments:  activation of p38

S990-p - TAO1 (human)
Modsite: SrSTsVTsQIsNGSH SwissProt Entrez-Gene
Orthologous residues
TAO1 (human): S990‑p, TAO1 iso3 (human): S842‑p, TAO1 (mouse): S990‑p, TAO1 (rat): S990‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ATM (human)
Downstream Regulation
Effect of modification (function):  activity, induced
Effect of modification (process):  cell cycle regulation
Comments:  activation of p38

S324-p - TAO3 (human)
Modsite: RNGPLNEsQEDEEDs SwissProt Entrez-Gene
Orthologous residues
TAO3 (human): S324‑p, TAO3 (mouse): S324‑p, TAO3 (rat): S324‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  293 (epithelial), HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  activity, induced
Effect of modification (process):  cell cycle regulation
Comments:  activation of p38