Curated Information
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Home > Curated Information Page > PubMed Id: 30782827
VerPlank JJS, Lokireddy S, Zhao J, Goldberg AL (2019) 26S Proteasomes are rapidly activated by diverse hormones and physiological states that raise cAMP and cause Rpn6 phosphorylation. Proc Natl Acad Sci U S A 30782827
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S14-p - PSMD11 (human)
Modsite: VEFQrAQsLLSTDrE SwissProt Entrez-Gene
Orthologous residues
PSMD11 (human): S14‑p, PSMD11 iso2 (human): S14‑p, PSMD11 (mouse): S14‑p, PSMD11 (rat): S92‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'muscle, skeletal', 293 (epithelial)
Cellular systems studied:  cell lines, primary cells
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKACA (human) transfection of constitutively active enzyme, pharmacological activator of upstream enzyme
KINASE PKACA (human) transfection of wild-type enzyme, genetic knockout/knockin of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
NKH_477 increase
exercise increase
muscle contraction increase
Downstream Regulation
Effect of modification (function):  activity, induced
Comments:  Increases proteosome activity

S14-p - PSMD11 (mouse)
Modsite: VEFQRAQsLLSTDRE SwissProt Entrez-Gene
Orthologous residues
PSMD11 (human): S14‑p, PSMD11 iso2 (human): S14‑p, PSMD11 (mouse): S14‑p, PSMD11 (rat): S92‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  heart, hepatocyte, mpkCCD (renal)
Cellular systems studied:  cell lines
Species studied:  mouse, rat
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKACA (human) transfection of wild-type enzyme, genetic knockout/knockin of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
NKH_477 increase
glucagon increase
racepinefrine increase
PP1 decrease
fasting increase
racepinefrine increase
desmopressin increase
Downstream Regulation
Effect of modification (function):  activity, induced
Comments:  Increases proteosome activity

S92-p - PSMD11 (rat)
Modsite: VEFQRAQsLLSTDRE SwissProt Entrez-Gene
Orthologous residues
PSMD11 (human): S14‑p, PSMD11 iso2 (human): S14‑p, PSMD11 (mouse): S14‑p, PSMD11 (rat): S92‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  heart
Cellular systems studied:  tissue
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
racepinefrine increase
exercise increase
muscle contraction increase