Curated Information
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Home > Curated Information Page > PubMed Id: 17371868
Nie L, Sasaki M, Maki CG (2007) Regulation of p53 nuclear export through sequential changes in conformation and ubiquitination. J Biol Chem 282, 14616-25 17371868
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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K101-ub - p53 (human)
Modsite: SSSVPsQkTYQGsYG SwissProt Entrez-Gene
Orthologous residues
p53 (human): K101‑ub, p53 iso2 (human): K101‑ub, p53 iso4 (human): K62‑ub, p53 (mouse): K98‑ub, p53 iso2 (mouse): K98‑ub, p53 (rat): K99‑ub, p53 (rabbit): K98‑ub, p53 (green monkey): K101‑ub
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  bone cancer
Relevant cell lines - cell types - tissues:  Saos-2 (bone cell), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  multiple sites mutants have been used
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE MDM2 (human) transfection of wild-type enzyme
Downstream Regulation
Effect of modification (function):  intracellular localization, protein degradation
Comments:  required for MDM2-mediated nuclear export

K120-ub - p53 (human)
Modsite: FLhSGTAkSVTCTyS SwissProt Entrez-Gene
Orthologous residues
p53 (human): K120‑ub, p53 iso2 (human): K120‑ub, p53 iso4 (human): K81‑ub, p53 (mouse): K117‑ub, p53 iso2 (mouse): K117‑ub, p53 (rat): K118‑ub, p53 (rabbit): K117‑ub, p53 (green monkey): K120‑ub
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  bone cancer
Relevant cell lines - cell types - tissues:  Saos-2 (bone cell), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  multiple sites mutants have been used
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE MDM2 (human) transfection of wild-type enzyme
Downstream Regulation
Effect of modification (function):  intracellular localization, protein degradation
Comments:  required for MDM2-mediated nuclear export

K132-ub - p53 (human)
Modsite: TySPALNkMFCQLAk SwissProt Entrez-Gene
Orthologous residues
p53 (human): K132‑ub, p53 iso2 (human): K132‑ub, p53 iso4 (human): K93‑ub, p53 (mouse): K129‑ub, p53 iso2 (mouse): K129‑ub, p53 (rat): K130‑ub, p53 (rabbit): K129‑ub, p53 (green monkey): K132‑ub
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  bone cancer
Relevant cell lines - cell types - tissues:  Saos-2 (bone cell), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  multiple sites mutants have been used
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE MDM2 (human) transfection of wild-type enzyme
Downstream Regulation
Effect of modification (function):  intracellular localization, protein degradation
Comments:  required for MDM2-mediated nuclear export

K139-ub - p53 (human)
Modsite: kMFCQLAkTCPVQLW SwissProt Entrez-Gene
Orthologous residues
p53 (human): K139‑ub, p53 iso2 (human): K139‑ub, p53 iso4 (human): K100‑ub, p53 (mouse): K136‑ub, p53 iso2 (mouse): K136‑ub, p53 (rat): K137‑ub, p53 (rabbit): K136‑ub, p53 (green monkey): K139‑ub
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  bone cancer
Relevant cell lines - cell types - tissues:  Saos-2 (bone cell), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  multiple sites mutants have been used
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE MDM2 (human) transfection of wild-type enzyme
Downstream Regulation
Effect of modification (function):  intracellular localization, protein degradation
Comments:  required for MDM2-mediated nuclear export

K164-ub - p53 (human)
Modsite: VRAMAIYkQSQHMTE SwissProt Entrez-Gene
Orthologous residues
p53 (human): K164‑ub, p53 iso2 (human): K164‑ub, p53 iso4 (human): K125‑ub, p53 (mouse): K161‑ub, p53 iso2 (mouse): K161‑ub, p53 (rat): K162‑ub, p53 (rabbit): K161‑ub, p53 (green monkey): K164‑ub
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  bone cancer
Relevant cell lines - cell types - tissues:  Saos-2 (bone cell), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  multiple sites mutants have been used
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE MDM2 (human) transfection of wild-type enzyme
Downstream Regulation
Effect of modification (function):  intracellular localization, protein degradation
Comments:  required for MDM2-mediated nuclear export

K370-ub - p53 (human)
Modsite: RAHsSHLkskkGQst SwissProt Entrez-Gene
Orthologous residues
p53 (human): K370‑ub, p53 iso2 (human): , p53 iso4 (human): K331‑ub, p53 (mouse): K367‑ub, p53 iso2 (mouse): , p53 (rat): K368‑ub, p53 (rabbit): K368‑ub, p53 (green monkey): K370‑ub
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  bone cancer
Relevant cell lines - cell types - tissues:  Saos-2 (bone cell), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  multiple sites mutants have been used
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE MDM2 (human) transfection of wild-type enzyme
Downstream Regulation
Effect of modification (function):  intracellular localization, protein degradation
Comments:  required for MDM2-mediated nuclear export

K372-ub - p53 (human)
Modsite: HsSHLkskkGQstsR SwissProt Entrez-Gene
Orthologous residues
p53 (human): K372‑ub, p53 iso2 (human): , p53 iso4 (human): K333‑ub, p53 (mouse): K369‑ub, p53 iso2 (mouse): , p53 (rat): K370‑ub, p53 (rabbit): K370‑ub, p53 (green monkey): K372‑ub
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  bone cancer
Relevant cell lines - cell types - tissues:  Saos-2 (bone cell), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  multiple sites mutants have been used
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE MDM2 (human) transfection of wild-type enzyme
Downstream Regulation
Effect of modification (function):  intracellular localization, protein degradation
Comments:  required for MDM2-mediated nuclear export

K373-ub - p53 (human)
Modsite: sSHLkskkGQstsRH SwissProt Entrez-Gene
Orthologous residues
p53 (human): K373‑ub, p53 iso2 (human): , p53 iso4 (human): K334‑ub, p53 (mouse): K370‑ub, p53 iso2 (mouse): , p53 (rat): K371‑ub, p53 (rabbit): K371‑ub, p53 (green monkey): K373‑ub
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  bone cancer
Relevant cell lines - cell types - tissues:  Saos-2 (bone cell), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  multiple sites mutants have been used
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE MDM2 (human) transfection of wild-type enzyme
Downstream Regulation
Effect of modification (function):  intracellular localization, protein degradation
Comments:  required for MDM2-mediated nuclear export

K381-ub - p53 (human)
Modsite: GQstsRHkkLMFktE SwissProt Entrez-Gene
Orthologous residues
p53 (human): K381‑ub, p53 iso2 (human): , p53 iso4 (human): K342‑ub, p53 (mouse): K378‑ub, p53 iso2 (mouse): , p53 (rat): K379‑ub, p53 (rabbit): K379‑ub, p53 (green monkey): K381‑ub
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  bone cancer
Relevant cell lines - cell types - tissues:  Saos-2 (bone cell), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  multiple sites mutants have been used
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE MDM2 (human) transfection of wild-type enzyme
Downstream Regulation
Effect of modification (function):  intracellular localization, protein degradation
Comments:  required for MDM2-mediated nuclear export

K382-ub - p53 (human)
Modsite: QstsRHkkLMFktEG SwissProt Entrez-Gene
Orthologous residues
p53 (human): K382‑ub, p53 iso2 (human): , p53 iso4 (human): K343‑ub, p53 (mouse): K379‑ub, p53 iso2 (mouse): , p53 (rat): K380‑ub, p53 (rabbit): K380‑ub, p53 (green monkey): K382‑ub
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  bone cancer
Relevant cell lines - cell types - tissues:  Saos-2 (bone cell), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  multiple sites mutants have been used
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE MDM2 (human) transfection of wild-type enzyme
Downstream Regulation
Effect of modification (function):  intracellular localization, protein degradation
Comments:  required for MDM2-mediated nuclear export

K386-ub - p53 (human)
Modsite: RHkkLMFktEGPDsD SwissProt Entrez-Gene
Orthologous residues
p53 (human): K386‑ub, p53 iso2 (human): , p53 iso4 (human): K347‑ub, p53 (mouse): K383‑ub, p53 iso2 (mouse): , p53 (rat): K384‑ub, p53 (rabbit): K384‑ub, p53 (green monkey): K386‑ub
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  bone cancer
Relevant cell lines - cell types - tissues:  Saos-2 (bone cell), U2OS (bone cell)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  multiple sites mutants have been used
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
UBIQUITIN LIGASE MDM2 (human) transfection of wild-type enzyme
Downstream Regulation
Effect of modification (function):  intracellular localization, protein degradation
Comments:  required for MDM2-mediated nuclear export