Staub O, et al. (1997) Regulation of stability and function of the epithelial Na+ channel (ENaC) by ubiquitination. EMBO J 16, 6325-36 9351815

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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K6-ub - ENaC-gamma (rat) ▲

Modsite: __MAPGEkIkAkIkk SwissProt Entrez-Gene Orthologous residues ENaC‑gamma (human): K6‑ub, ENaC‑gamma (mouse): K6‑ub, ENaC‑gamma (rat): K6‑ub Characterization Methods used to characterize site in vivo:  electrophoretic mobility shift, immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast), COS7 (fibroblast), MDCK (epithelial), oocyte Cellular systems studied:  cell lines Species studied:  dog, frog, green monkey, human, mouse Downstream Regulation Effect of modification (function):  intracellular localization, protein degradation, receptor recycling, inhibited

K8-ub - ENaC-gamma (rat) ▲

Modsite: MAPGEkIkAkIkkNL SwissProt Entrez-Gene Orthologous residues ENaC‑gamma (human): K8‑ub, ENaC‑gamma (mouse): K8‑ub, ENaC‑gamma (rat): K8‑ub Characterization Methods used to characterize site in vivo:  electrophoretic mobility shift, immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast), COS7 (fibroblast), MDCK (epithelial), oocyte Cellular systems studied:  cell lines Species studied:  dog, frog, green monkey, human, mouse Downstream Regulation Effect of modification (function):  intracellular localization, protein degradation, receptor recycling, inhibited

K10-ub - ENaC-gamma (rat) ▲

Modsite: PGEkIkAkIkkNLPV SwissProt Entrez-Gene Orthologous residues ENaC‑gamma (human): K10‑ub, ENaC‑gamma (mouse): K10‑ub, ENaC‑gamma (rat): K10‑ub Characterization Methods used to characterize site in vivo:  electrophoretic mobility shift, immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast), COS7 (fibroblast), MDCK (epithelial), oocyte Cellular systems studied:  cell lines Species studied:  dog, frog, green monkey, human, mouse Downstream Regulation Effect of modification (function):  intracellular localization, protein degradation, receptor recycling, inhibited

K12-ub - ENaC-gamma (rat) ▲

Modsite: EkIkAkIkkNLPVRG SwissProt Entrez-Gene Orthologous residues ENaC‑gamma (human): K12‑ub, ENaC‑gamma (mouse): K12‑ub, ENaC‑gamma (rat): K12‑ub Characterization Methods used to characterize site in vivo:  electrophoretic mobility shift, immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast), COS7 (fibroblast), MDCK (epithelial), oocyte Cellular systems studied:  cell lines Species studied:  dog, frog, green monkey, human, mouse Downstream Regulation Effect of modification (function):  intracellular localization, protein degradation, receptor recycling, inhibited

K13-ub - ENaC-gamma (rat) ▲

Modsite: kIkAkIkkNLPVRGP SwissProt Entrez-Gene Orthologous residues ENaC‑gamma (human): K13‑ub, ENaC‑gamma (mouse): K13‑ub, ENaC‑gamma (rat): K13‑ub Characterization Methods used to characterize site in vivo:  electrophoretic mobility shift, immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast), COS7 (fibroblast), MDCK (epithelial), oocyte Cellular systems studied:  cell lines Species studied:  dog, frog, green monkey, human, mouse Downstream Regulation Effect of modification (function):  intracellular localization, protein degradation, receptor recycling, inhibited

K26-ub - ENaC-gamma (rat) ▲

Modsite: GPQAPTIkDLMHWYC SwissProt Entrez-Gene Orthologous residues ENaC‑gamma (human): K26‑ub, ENaC‑gamma (mouse): K26‑ub, ENaC‑gamma (rat): K26‑ub Characterization Methods used to characterize site in vivo:  electrophoretic mobility shift, immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast), COS7 (fibroblast), MDCK (epithelial), oocyte Cellular systems studied:  cell lines Species studied:  dog, frog, green monkey, human, mouse