Curated Information
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Home > Curated Information Page > PubMed Id: 17613530
Li J, et al. (2007) Protein kinase C phosphorylation disrupts Na+/H+ exchanger regulatory factor 1 autoinhibition and promotes cystic fibrosis transmembrane conductance regulator macromolecular assembly. J Biol Chem 282, 27086-99 17613530
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S162-p - NHERF (human)
Modsite: CTMKKGPsGYGFNLH SwissProt Entrez-Gene
Orthologous residues
NHERF (human): S162‑p, NHERF (mouse): N157‑p, NHERF (rat): N159‑p, NHERF (rabbit): N162‑p, NHERF (dog): N169‑p
Characterization
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCA (human)

S339-p - NHERF (human)
Modsite: ERAHQKRsSKRAPQM SwissProt Entrez-Gene
Orthologous residues
NHERF (human): S339‑p, NHERF (mouse): S336‑p, NHERF (rat): S337‑p, NHERF (rabbit): S339‑p, NHERF (dog): S348‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  lung cancer
Relevant cell lines - cell types - tissues:  Calu-3 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCA (human)
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
CFTR (human) Induces in vitro, co-immunoprecipitation

S340-p - NHERF (human)
Modsite: RAHQKRSsKRAPQMD SwissProt Entrez-Gene
Orthologous residues
NHERF (human): S340‑p, NHERF (mouse): S337‑p, NHERF (rat): S338‑p, NHERF (rabbit): S340‑p, NHERF (dog): S349‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  lung cancer
Relevant cell lines - cell types - tissues:  Calu-3 (pulmonary)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCA (human)
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
CFTR (human) Induces in vitro, co-immunoprecipitation