Curated Information
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Home > Curated Information Page > PubMed Id: 19935711
Treins C, et al. (2010) Rictor is a novel target of p70 S6 kinase-1. Oncogene 29, 1003-16 19935711
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S473-p - Akt1 (human)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PI-103 decrease
LY294002 decrease
rapamycin no change compared to control
insulin increase
rapamycin insulin no effect upon treatment-induced increase
amino_acids no change compared to control

T412-p - p70S6K (human)
Modsite: NQVFLGFtyVAPsVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PI-103 decrease
LY294002 decrease
rapamycin decrease
amino_acids increase
rapamycin amino_acids inhibit treatment-induced increase
insulin increase
rapamycin insulin inhibit treatment-induced increase

S21-p - RICTOR (human)
Modsite: RVRGRNDsGEENVPL SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): S21‑p, RICTOR iso3 (human): S21‑p, RICTOR iso4 (human): S21‑p, RICTOR (mouse): S21‑p, RICTOR (rat): S6‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

S1035-p - RICTOR (human)
Modsite: sTssRHNsEsESVPS SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): S1035‑p, RICTOR iso3 (human): S1035‑p, RICTOR iso4 (human): , RICTOR (mouse): S1035‑p, RICTOR (rat): S1020‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

T1135-p - RICTOR (human)
Modsite: NRRIRtLtEPsVDFN SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): T1135‑p, RICTOR iso3 (human): T1135‑p, RICTOR iso4 (human): , RICTOR (mouse): T1135‑p, RICTOR (rat): T1120‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE p70S6K (human)
KINASE P70S6KB (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE p70S6K (human) genetic knockout/knockin of upstream enzyme, siRNA inhibition of enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PI-103 decrease
LY294002 decrease
rapamycin decrease
amino_acids increase
rapamycin amino_acids inhibit treatment-induced increase
insulin increase
rapamycin insulin inhibit treatment-induced increase
EGF increase
serum increase
calyculin_A increase
U0126 no change compared to control
mTOR (human) increase mTOR siRNA decreases
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 epsilon (human) Induces co-immunoprecipitation

S1138-p - RICTOR (human)
Modsite: IRtLtEPsVDFNHsD SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): S1138‑p, RICTOR iso3 (human): S1138‑p, RICTOR iso4 (human): , RICTOR (mouse): S1138‑p, RICTOR (rat): S1123‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Comments:  ambiguous

T1172-p - RICTOR (human)
Modsite: GNkHIEDtGstPsIG SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): T1172‑p, RICTOR iso3 (human): T1172‑p, RICTOR iso4 (human): , RICTOR (mouse): A1171‑p, RICTOR (rat): T1157‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  ambiguous

S1174-p - RICTOR (human)
Modsite: kHIEDtGstPsIGEN SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): S1174‑p, RICTOR iso3 (human): S1174‑p, RICTOR iso4 (human): , RICTOR (mouse): S1173‑p, RICTOR (rat): S1159‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  ambiguous

T1175-p - RICTOR (human)
Modsite: HIEDtGstPsIGEND SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): T1175‑p, RICTOR iso3 (human): T1175‑p, RICTOR iso4 (human): , RICTOR (mouse): T1174‑p, RICTOR (rat): T1160‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  ambiguous

S1177-p - RICTOR (human)
Modsite: EDtGstPsIGENDLk SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): S1177‑p, RICTOR iso3 (human): S1177‑p, RICTOR iso4 (human): , RICTOR (mouse): S1176‑p, RICTOR (rat): S1162‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

S1219-p - RICTOR (human)
Modsite: HMKIRsQsFNtDttt SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): S1219‑p, RICTOR iso3 (human): S1219‑p, RICTOR iso4 (human): , RICTOR (mouse): S1218‑p, RICTOR (rat): S1204‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  ambiguous

T1222-p - RICTOR (human)
Modsite: IRsQsFNtDtttsGI SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): T1222‑p, RICTOR iso3 (human): T1222‑p, RICTOR iso4 (human): , RICTOR (mouse): T1221‑p, RICTOR (rat): T1207‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  ambiguous

T1224-p - RICTOR (human)
Modsite: sQsFNtDtttsGIss SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): T1224‑p, RICTOR iso3 (human): T1224‑p, RICTOR iso4 (human): , RICTOR (mouse): T1223‑p, RICTOR (rat): T1209‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  ambiguous

T1225-p - RICTOR (human)
Modsite: QsFNtDtttsGIssM SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): T1225‑p, RICTOR iso3 (human): T1225‑p, RICTOR iso4 (human): , RICTOR (mouse): T1224‑p, RICTOR (rat): T1210‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  ambiguous

T1226-p - RICTOR (human)
Modsite: sFNtDtttsGIssMS SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): T1226‑p, RICTOR iso3 (human): T1226‑p, RICTOR iso4 (human): , RICTOR (mouse): T1225‑p, RICTOR (rat): T1211‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  ambiguous

S1227-p - RICTOR (human)
Modsite: FNtDtttsGIssMSs SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): S1227‑p, RICTOR iso3 (human): S1227‑p, RICTOR iso4 (human): , RICTOR (mouse): S1226‑p, RICTOR (rat): S1212‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  ambiguous

S1230-p - RICTOR (human)
Modsite: DtttsGIssMSssPS SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): S1230‑p, RICTOR iso3 (human): S1230‑p, RICTOR iso4 (human): , RICTOR (mouse): S1229‑p, RICTOR (rat): S1215‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  ambiguous

S1231-p - RICTOR (human)
Modsite: tttsGIssMSssPSR SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): S1231‑p, RICTOR iso3 (human): S1231‑p, RICTOR iso4 (human): , RICTOR (mouse): S1230‑p, RICTOR (rat): S1216‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  ambiguous

S1235-p - RICTOR (human)
Modsite: GIssMSssPSRETVG SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): S1235‑p, RICTOR iso3 (human): S1235‑p, RICTOR iso4 (human): , RICTOR (mouse): S1234‑p, RICTOR (rat): S1220‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

S1292-p - RICTOR (human)
Modsite: VsLVPPGssHtLPRR SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): S1292‑p, RICTOR iso3 (human): S1292‑p, RICTOR iso4 (human): , RICTOR (mouse): S1291‑p, RICTOR (rat): S1277‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  ambiguous

S1293-p - RICTOR (human)
Modsite: sLVPPGssHtLPRRA SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): S1293‑p, RICTOR iso3 (human): S1293‑p, RICTOR iso4 (human): , RICTOR (mouse): S1292‑p, RICTOR (rat): S1278‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  ambiguous

T1295-p - RICTOR (human)
Modsite: VPPGssHtLPRRAQs SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): T1295‑p, RICTOR iso3 (human): T1295‑p, RICTOR iso4 (human): , RICTOR (mouse): T1294‑p, RICTOR (rat): T1280‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  ambiguous

S1302-p - RICTOR (human)
Modsite: tLPRRAQsLkAPsIA SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): S1302‑p, RICTOR iso3 (human): S1302‑p, RICTOR iso4 (human): , RICTOR (mouse): S1301‑p, RICTOR (rat): S1287‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

S1385-p - RICTOR (human)
Modsite: SRFMkALsyAsLDkE SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): S1385‑p, RICTOR iso3 (human): S1409‑p, RICTOR iso4 (human): , RICTOR (mouse): S1384‑p, RICTOR (rat): S1370‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  ambiguous

Y1386-p - RICTOR (human)
Modsite: RFMkALsyAsLDkED SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): Y1386‑p, RICTOR iso3 (human): Y1410‑p, RICTOR iso4 (human): , RICTOR (mouse): Y1385‑p, RICTOR (rat): Y1371‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  ambiguous

S1388-p - RICTOR (human)
Modsite: MkALsyAsLDkEDLL SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): S1388‑p, RICTOR iso3 (human): S1412‑p, RICTOR iso4 (human): , RICTOR (mouse): S1387‑p, RICTOR (rat): S1373‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  ambiguous

S1414-p - RICTOR (human)
Modsite: sSVRsMVssAtyGGs SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): S1414‑p, RICTOR iso3 (human): S1438‑p, RICTOR iso4 (human): , RICTOR (mouse): S1413‑p, RICTOR (rat): S1399‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  ambiguous

S1415-p - RICTOR (human)
Modsite: SVRsMVssAtyGGsD SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): S1415‑p, RICTOR iso3 (human): S1439‑p, RICTOR iso4 (human): , RICTOR (mouse): S1414‑p, RICTOR (rat): S1400‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  ambiguous

T1417-p - RICTOR (human)
Modsite: RsMVssAtyGGsDDY SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): T1417‑p, RICTOR iso3 (human): T1441‑p, RICTOR iso4 (human): , RICTOR (mouse): T1416‑p, RICTOR (rat): T1402‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  ambiguous

Y1418-p - RICTOR (human)
Modsite: sMVssAtyGGsDDYI SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): Y1418‑p, RICTOR iso3 (human): Y1442‑p, RICTOR iso4 (human): , RICTOR (mouse): Y1417‑p, RICTOR (rat): Y1403‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  ambiguous

S1421-p - RICTOR (human)
Modsite: ssAtyGGsDDYIGLA SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): S1421‑p, RICTOR iso3 (human): S1445‑p, RICTOR iso4 (human): , RICTOR (mouse): S1420‑p, RICTOR (rat): S1406‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  ambiguous

S1470-p - RICTOR (human)
Modsite: HDAGGLPsGtGGLVk SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): S1470‑p, RICTOR iso3 (human): S1494‑p, RICTOR iso4 (human): , RICTOR (mouse): S1469‑p, RICTOR (rat): S1455‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  ambiguous

T1472-p - RICTOR (human)
Modsite: AGGLPsGtGGLVkNs SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): T1472‑p, RICTOR iso3 (human): T1496‑p, RICTOR iso4 (human): , RICTOR (mouse): A1471‑p, RICTOR (rat): T1457‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  ambiguous

S1479-p - RICTOR (human)
Modsite: tGGLVkNsFHLLRQQ SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): S1479‑p, RICTOR iso3 (human): S1503‑p, RICTOR iso4 (human): , RICTOR (mouse): S1478‑p, RICTOR (rat): S1464‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

S1591-p - RICTOR (human)
Modsite: GsASSTkstELLLGV SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): S1591‑p, RICTOR iso3 (human): S1615‑p, RICTOR iso4 (human): , RICTOR (mouse): S1591‑p, RICTOR (rat): S1577‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  ambiguous

T1592-p - RICTOR (human)
Modsite: sASSTkstELLLGVk SwissProt Entrez-Gene
Orthologous residues
RICTOR (human): T1592‑p, RICTOR iso3 (human): T1616‑p, RICTOR iso4 (human): , RICTOR (mouse): T1592‑p, RICTOR (rat): T1578‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  ambiguous

S235-p - S6 (human)
Modsite: IAKRRRLssLRAsts SwissProt Entrez-Gene
Orthologous residues
S6 (human): S235‑p, S6 (mouse): S235‑p, S6 (rat): S235‑p, S6 (fruit fly): A234‑p, S6 (cow): S235‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PI-103 decrease
LY294002 decrease
rapamycin decrease
amino_acids increase
rapamycin amino_acids inhibit treatment-induced increase
insulin increase
rapamycin insulin inhibit treatment-induced increase
RICTOR (mouse) increase Rictor wild-type
rapamycin RICTOR (mouse) inhibit treatment-induced increase

S236-p - S6 (human)
Modsite: AKRRRLssLRAstsK SwissProt Entrez-Gene
Orthologous residues
S6 (human): S236‑p, S6 (mouse): S236‑p, S6 (rat): S236‑p, S6 (fruit fly): S235‑p, S6 (cow): S236‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PI-103 decrease
LY294002 decrease
rapamycin decrease
amino_acids increase
rapamycin amino_acids inhibit treatment-induced increase
insulin increase
rapamycin insulin inhibit treatment-induced increase
RICTOR (mouse) increase Rictor wild-type
rapamycin RICTOR (mouse) inhibit treatment-induced increase

T308-p - Akt1 (mouse)
Modsite: KDGAtMKtFCGtPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum RICTOR (human) increase Rictor +/+ MEFs
serum RICTOR (mouse) decrease Rictor -/- increases
RICTOR (mouse) no change compared to control
rapamycin RICTOR (mouse) increase

T450-p - Akt1 (mouse)
Modsite: TAQMITItPPDQDDS SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T450‑p, Akt1 iso2 (human): T388‑p, Akt1 (mouse): T450‑p, Akt1 (rat): T450‑p, Akt1 (fruit fly): T565‑p, Akt1 (cow): T450‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum RICTOR (human) no change compared to control Rictor +/+ MEFs
serum RICTOR (human) no change compared to control Rictor -/- no change
RICTOR (mouse) increase Rictor wild-type
rapamycin RICTOR (mouse) inhibit treatment-induced increase

S473-p - Akt1 (mouse)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum RICTOR (human) increase Rictor +/+ MEFs
serum RICTOR (human) no change compared to control Rictor -/- no change
RICTOR (mouse) no change compared to control
rapamycin RICTOR (mouse) increase

T32-p - FOXO3A (mouse)
Modsite: QSRPRSCtWPLQRPE SwissProt Entrez-Gene
Orthologous residues
FOXO3A (human): T32‑p, FOXO3A (mouse): T32‑p, FOXO3A (rat): T32‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum RICTOR (mouse) decrease Rictor -/- increases

S21-p - GSK3A (mouse)
Modsite: sGRARtssFAEPGGG SwissProt Entrez-Gene
Orthologous residues
GSK3A (human): S21‑p, GSK3A (mouse): S21‑p, GSK3A (rat): S21‑p, GSK3A (cow): S21‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum RICTOR (human) increase Rictor +/+ MEFs
serum RICTOR (mouse) decrease Rictor -/- increases

S9-p - GSK3B (mouse)
Modsite: SGRPRttsFAEsCKP SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S9‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum RICTOR (human) increase Rictor +/+ MEFs
serum RICTOR (mouse) decrease Rictor -/- increases

T346-p - NDRG1 (mouse)
Modsite: GtRsRsHtsEGPRsR SwissProt Entrez-Gene
Orthologous residues
NDRG1 (human): T346‑p, NDRG1 iso2 (human): T280‑p, NDRG1 iso3 (human): T265‑p, NDRG1 (mouse): T346‑p, NDRG1 (rat): T346‑p, NDRG1 (cow): T346‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum RICTOR (human) increase Rictor +/+ MEFs
serum RICTOR (human) no change compared to control Rictor -/- no change
RICTOR (mouse) increase Rictor wild-type
rapamycin RICTOR (mouse) inhibit treatment-induced increase
RICTOR (mouse) no change compared to control
rapamycin RICTOR (mouse) increase

T412-p - p70S6K (mouse)
Modsite: NQVFLGFtYVAPSVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum RICTOR (human) increase Rictor +/+ MEFs
serum RICTOR (mouse) decrease Rictor -/- increases

T638-p - PKCA (mouse)
Modsite: TRGQPVLtPPDQLVI SwissProt Entrez-Gene
Orthologous residues
PKCA (human): T638‑p, PKCA (mouse): T638‑p, PKCA (rat): T638‑p, PKCA (cow): T638‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum RICTOR (human) no change compared to control Rictor +/+ MEFs
serum RICTOR (human) no change compared to control Rictor -/- no change
RICTOR (mouse) increase
rapamycin RICTOR (mouse) no effect upon treatment-induced increase

Y658-p - PKCA (mouse)
Modsite: sDFEGFsyVNPQFVH SwissProt Entrez-Gene
Orthologous residues
PKCA (human): Y658‑p, PKCA (mouse): Y658‑p, PKCA (rat): Y658‑p, PKCA (cow): Y658‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum RICTOR (human) no change compared to control Rictor +/+ MEFs
serum RICTOR (human) no change compared to control Rictor -/- no change
RICTOR (mouse) increase
rapamycin RICTOR (mouse) no effect upon treatment-induced increase

T642-p - PKCB (mouse)
Modsite: TRQPVELtPTDKLFI SwissProt Entrez-Gene
Orthologous residues
PKCB (human): T642‑p, PKCB iso2 (human): T641‑p, PKCB (mouse): T642‑p, PKCB iso2 (mouse): T641‑p, PKCB (rat): T642‑p, PKCB iso2 (rat): T641‑p, PKCB (cow): T641‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum RICTOR (human) no change compared to control Rictor -/- no change
RICTOR (mouse) increase
rapamycin RICTOR (mouse) no effect upon treatment-induced increase

T1465-p - TSC2 (mouse)
Modsite: GLRPRGYtISDSAPS SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): T1462‑p, TSC2 iso2 (human): T1419‑p, TSC2 iso3 (human): T1418‑p, TSC2 iso4 (human): T1439‑p, TSC2 (mouse): T1465‑p, TSC2 iso6 (mouse): T1443‑p, TSC2 (rat): T1466‑p, TSC2 iso2 (rat): T1423‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum RICTOR (human) increase Rictor +/+ MEFs
serum RICTOR (mouse) decrease Rictor -/- increases